Literature summary extracted from

Saeed, H.M.; Alanazi, M.S.; Abduljaleel, Z.; Al-Amri, A.; Khan, Z.
Molecular cloning, sequence analysis and expression in Escherichia coli of Camelus dromedarius glucose-6-phosphate dehydrogenase cDNA (2012), Protein Expr. Purif., 83, 190-197.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.49	DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Camelus dromedarius

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.1.49	Mg2+	required	Camelus dromedarius

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.49	63000	-	x * 63000, SDS-PAGE	Camelus dromedarius

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.49	D-glucose 6-phosphate + NADP+	Camelus dromedarius	-	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.49	Camelus dromedarius	G1EHI3	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.49	recombinant His-tagged enzyme 95.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Camelus dromedarius

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.49	289.6	-	purified recombinant His-tagged enzyme, pH 7.5, 25°C	Camelus dromedarius

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.49	D-glucose 6-phosphate + NADP+	-	Camelus dromedarius	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.49	?	x * 63000, SDS-PAGE	Camelus dromedarius
1.1.1.49	More	structure modeling	Camelus dromedarius

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.49	G6PD	-	Camelus dromedarius

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.49	25	-	assay at	Camelus dromedarius

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.49	7.5	-	assay at	Camelus dromedarius

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.49	NADP+	the enzyme has two NADP+ binding sites, one is the structural NADP+ and the second is the catalytic NADP+. The structural NADP+ domain adopt a classic beta-alpha-beta dinucleotide fold with the fingerprint sequence GASGDLA at the tight turn following the first beta-strand	Camelus dromedarius

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Release 2023.1 (January 2023)