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Literature summary extracted from
- Purification of rat kidney glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase enzymes using 2,5-ADP Sepharose 4B affinity in a single chromatography step (2012), Protein Expr. Purif., 81, 1-4.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.43 | 56000 | - |
2 * 56000, SDS-PAGE | Rattus norvegicus |
| 1.1.1.43 | 110000 | - |
gel filtration | Rattus norvegicus |
| 1.1.1.49 | 68000 | - |
2 * 68000, SDS-PAGE | Rattus norvegicus |
| 1.1.1.49 | 144000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | Rattus norvegicus | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.43 | Rattus norvegicus | - |
- |
- |
| 1.1.1.49 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.43 | ammonium sulfate precipitation, 2,5-ADP Sepharose 4B column chromatography, and Sephadex G-25 gel filtration | Rattus norvegicus |
| 1.1.1.49 | native enzyme 531fold from kidney, co-purification with 6-phosphogluconate dehydrogenase and glutathione reductase in one chromatographic step by 2',5'-ADP affinity chromatography by using different elution buffers, method development and optimization, overview | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.43 | kidney | - |
Rattus norvegicus | - |
| 1.1.1.49 | kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.43 | additional information | - |
the homogenate has a specific activity of 0.05 EU/mg, after 484fold purification the specific activity is 25 EU/mg | Rattus norvegicus |
| 1.1.1.49 | 32 | - |
purified enzyme, pH and temperature not specified in the publication | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | - |
Rattus norvegicus | 6-phospho-2-dehydro-D-gluconate + NAD(P)H + H+ | - |
? | |
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | - |
Rattus norvegicus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.43 | homodimer | 2 * 56000, SDS-PAGE | Rattus norvegicus |
| 1.1.1.49 | dimer | 2 * 68000, SDS-PAGE | Rattus norvegicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.43 | 6-phosphogluconate dehydrogenase | - |
Rattus norvegicus |
| 1.1.1.43 | 6PGD | - |
Rattus norvegicus |
| 1.1.1.49 | G6PD | - |
Rattus norvegicus |
| 1.1.1.49 | glucose 6-phosphate dehydrogenase | - |
Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | NADP+ | - |
Rattus norvegicus | |
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