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Literature summary extracted from

  • Shi, R.; McDonald, L.; Cui, Q.; Matte, A.; Cygler, M.; Ekiel, I.
    Structural and mechanistic insight into covalent substrate binding by Escherichia coli dihydroxyacetone kinase (2011), Proc. Natl. Acad. Sci. USA, 108, 1302-1307.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.29 DhaK-DhaL complex bound to ADP anddihydroxyacetone, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 3.5 M sodium formate or 0.1 M sodium citrate pH 5.6, 20% (w/v)PEG 8000, at 19°C Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.29 D109A inactive Escherichia coli
2.7.1.29 D109N inactive Escherichia coli
2.7.1.29 H128A inactive Escherichia coli
2.7.1.29 H128K inactive Escherichia coli
2.7.1.29 H56A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
2.7.1.29 H56N the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.29 0.008
-
 dihydroxyacetone apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.7.1.29 0.302
-
 dihydroxyacetone apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.7.1.29 2.2
-
 dihydroxyacetone apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.29 Escherichia coli P76015 subunit DhaK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.29 nickel affinity column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.29 ATP + dihydroxyacetone
-
 Escherichia coli ADP + dihydroxyacetone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.29 heterotetramer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.29 DHA kinase
-
 Escherichia coli
2.7.1.29 DhaK subunit Escherichia coli
2.7.1.29 DhaL subunit Escherichia coli
2.7.1.29 dihydroxyacetone kinase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.29 1.6
-
 dihydroxyacetone apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.7.1.29 3.72
-
 dihydroxyacetone apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.7.1.29 8.45
-
 dihydroxyacetone apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.29 1.7
-
 dihydroxyacetone apparent value, mutant enzyme H56N, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.7.1.29 5.3
-
 dihydroxyacetone apparent value, mutant enzyme H56A, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli
2.7.1.29 954
-
 dihydroxyacetone apparent value, Km above 0.008 mM, wild type enzyme, in 50 mM potassium phosphate pH 7.5, at 22°C Escherichia coli