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Literature summary extracted from

  • Yang, W.; Pollard, M.; Li-Beisson, Y.; Beisson, F.; Feig, M.; Ohlrogge, J.
    A distinct type of glycerol-3-phosphate acyltransferase with sn-2 preference and phosphatase activity producing 2-monoacylglycerol (2010), Proc. Natl. Acad. Sci. USA, 107, 12040-12045.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.198 cloned in yeast microsomes and wheat germ cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.198 membrane modeling data indicate this localisation Arabidopsis thaliana 16020
-
3.1.3.B13 membrane modeling data indicate Arabidopsis thaliana 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.B13 Mg2+ necessary for phosphatase activity Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.198 Arabidopsis thaliana O80437
-
-
2.3.1.198 Arabidopsis thaliana Q9CAY3
-
-
2.3.1.198 Arabidopsis thaliana Q9LMM0
-
-
3.1.3.B13 Arabidopsis thaliana O80437
-
-
3.1.3.B13 Arabidopsis thaliana Q9LMM0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.198 sn-glycerol 3-phosphate + (9Z)-17-carboxyheptadec-9-enoyl-CoA (9Z)-17-carboxyheptadec-9-enoyl-CoA = C18:1 DCA-CoA Arabidopsis thaliana CoA + 1-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate + 2-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate ((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate = C18:1 DCA-LPA, ratio of sn-2:sn-1-labeled products is 5:1 or greater ?
2.3.1.198 sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA 15-carboxypentadecanoyl-CoA = C16:0 DCA-CoA Arabidopsis thaliana CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate (15-carboxypentadecanoyl)-sn-glycerol 3-phosphate = C16:0 DCA-LPA, ratio of sn-2:sn-1-labeled products is 5:1 or greater ?
2.3.1.198 sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA 15-carboxypentadecanoyl-CoA = C16:0 DCA-CoA Arabidopsis thaliana CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate (15-carboxypentadecanoyl)-sn-glycerol 3-phosphate = C16:0 DCA-LPA, sn-2:sn-1-labeled products is 2:1 ?
3.1.3.B13 2-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate + H2O ((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate = C18:1 DCA-LPA Arabidopsis thaliana 2-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol + phosphate
-
 ?
3.1.3.B13 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + H2O (15-carboxypentadecanoyl)-sn-glycerol 3-phosphate = C16:0 DCA-LPA Arabidopsis thaliana 2-(15-carboxypentadecanoyl)-sn-glycerol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.198 GPAT4 additional phosphatase activity Arabidopsis thaliana
2.3.1.198 GPAT5 no additional phosphatase activity Arabidopsis thaliana
2.3.1.198 GPAT6 additional phosphatase activity Arabidopsis thaliana
3.1.3.B13 GPAT4 additional acyltransferase activity Arabidopsis thaliana
3.1.3.B13 GPAT6 additional acyltransferase activity Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.198 20
-
 activity assay at room temperature Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.198 7.5
-
 activity assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.3.1.198 physiological function The enzyme is involved in cutin and suberin biosynthesis in plants. The common acyl monomer substrates (as CoA esters) are omega-hydroxy fatty acids (omega-OHFAs) and alpha,omega-dicarboxylic acids (DCAs). Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols (2-MAGs). Arabidopsis thaliana
2.3.1.198 physiological function The enzyme is involved in cutin and suberin biosynthesis in plants. The common acyl monomer substrates (as CoA esters) are omega-hydroxy fatty acids and alpha,omega-dicarboxylic acids. Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols (2-MAGs). Arabidopsis thaliana
3.1.3.B13 physiological function The enzyme is involved in cutin biosynthesis in plants. The enzyme is bifunctional and has an additional acyltransferase activity. Arabidopsis thaliana