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Literature summary extracted from

  • Tori, K.; Ishino, S.; Kiyonari, S.; Tahara, S.; Ishino, Y.
    A novel single-strand specific 3'-5' exonuclease found in the hyperthermophilic archaeon, Pyrococcus furiosus (2013), PLoS One, 8, e58497.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.11.1 expression in Escherichia coli Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.11.1 Mg2+ dependent upon a divalent cation, and Mg2+ is the most preferable Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.1 25596
-
 3 * 25596, calculated from sequence Pyrococcus furiosus
3.1.11.1 73400
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.1 Pyrococcus furiosus Q8TZE9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.1
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.1 additional information no endonuclease activity with either ssDNA or dsDNA Pyrococcus furiosus ?
-
 ?
3.1.11.1 single-stranded oligodeoxyribonucleotide + H2O excises the DNA at every two nucleotides from the 3' to 5' direction. The enzyme needs to grasp a 4–6 nt-long DNA strand to express its nucleolytic activity. The homo dT 30 nt-long homooligomer is the most preferable substrate for PfuExo I Pyrococcus furiosus single-stranded oligodeoxyribonucleotide + nucleoside 5'-monophosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.1 trimer 3 * 25596, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.1.11.1 PF2046
-
 Pyrococcus furiosus
3.1.11.1 PfuExo I
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.11.1 8.8
-
 assay at Pyrococcus furiosus