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Literature summary extracted from

  • Mittal, P.; Karthikeyan, S.; Chakraborti, P.K.
    Amino acids involved in polyphosphate synthesis and its mobilization are distinct in polyphosphate kinase-1 from Mycobacterium tuberculosis (2011), PLoS ONE, 6, e27398.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 A615S the mutant exhibits about wild type activity for ATP synthesis and polyphosphate synthesis Mycobacterium tuberculosis
2.7.4.1 E681A the mutant exhibits less than 5% activity for ATP synthesis and less than 30% activity for polyphosphate synthesis compared o the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 H150A the mutation causes PPK1 protein deficient but not defective in autophosphorylation and severely affects ATP or polyphosphate synthesis Mycobacterium tuberculosis
2.7.4.1 H150Q the mutation causes PPK1 protein deficient but not defective in autophosphorylation and mildly affects ATP or polyphosphate synthesis Mycobacterium tuberculosis
2.7.4.1 H480A the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 H480Q the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 H491A inactive Mycobacterium tuberculosis
2.7.4.1 H491Q inactive Mycobacterium tuberculosis
2.7.4.1 H510Q the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 N515A the mutant exhibits less than 2% activity for ATP synthesis and about 65% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 R431A the mutant exhibits less than 5% activity for ATP synthesis and about 78% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 R461A the mutant exhibits less than 2% activity for ATP synthesis and about 76% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 R624A the mutant exhibits less than 2% activity for ATP synthesis and about 65% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 R654A the mutant exhibits less than 1% activity for ATP synthesis and about 90% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 S668A the mutant exhibits less than 5% activity for ATP synthesis and less than 30% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 Y524A the mutant exhibits less than 10% activity for ATP synthesis and less than 20% activity for polyphosphate synthesis compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.16
-
 ADP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 0.176
-
 ADP mutant H510Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 0.18
-
 ADP mutant H480Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 0.2
-
 ADP mutant H480A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 1.35
-
 ATP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 1.38
-
 ATP mutant H480Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 1.47
-
 ATP mutant H480A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 1.5 2 ATP mutant H510Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 2
-
 ADP mutant Y524A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 5.2
-
 ATP mutant H510A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 6.8
-
 ADP mutant H510A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 8
-
 ADP isoform PPK2, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 11.3
-
 GDP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 13
-
 ATP mutant Y524A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ necessary for activity Mycobacterium tuberculosis
2.7.4.1 Mn2+ necessary for activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 87000
-
 2 * 87000, isoform PPK1, SDS-PAGE Mycobacterium tuberculosis
2.7.4.1 197000
-
 isoform PPK1, gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Mycobacterium tuberculosis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.4.1 phosphoprotein the protein is capable of autophosphorylation. Only His-491 is autophosphorylated and crucial for any enzymatic activity Mycobacterium tuberculosis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + (phosphate)20
-
 Mycobacterium tuberculosis ATP + (phosphate)19
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Mycobacterium tuberculosis ADP + (phosphate)n+1
-
 r
2.7.4.1 dADP + (phosphate)20
-
 Mycobacterium tuberculosis dATP + (phosphate)19
-
 r
2.7.4.1 GDP + (phosphate)20 isoform PPK1 affinity for the ADP as a substrate is about 70fold high as opposed to GDP Mycobacterium tuberculosis GTP + (phosphate)19
-
 r
2.7.4.1 UDP + (phosphate)20
-
 Mycobacterium tuberculosis UTP + (phosphate)19
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.1 homodimer 2 * 87000, isoform PPK1, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyphosphate kinase-1 isoform Mycobacterium tuberculosis
2.7.4.1 polyphosphate kinase-2 isoform Mycobacterium tuberculosis
2.7.4.1 PPK1 isoform Mycobacterium tuberculosis
2.7.4.1 PPK2 isoform Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 44.6
-
 ATP mutant H510Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 45
-
 ATP mutant H480Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 45.6
-
 ATP mutant H480A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 46.9
-
 ATP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 55
-
 ATP mutant Y524A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 55.4
-
 ATP mutant H510A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 210
-
 ADP isoform PPK2, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 332
-
 ADP mutant H510A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 622
-
 ADP mutant H510Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 645
-
 GDP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 943
-
 ADP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 950
-
 ADP mutant H480A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 971
-
 ADP mutant H480Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 994
-
 ADP mutant Y524A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.1 4.2
-
 ATP mutant Y524A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 10.5
-
 ATP mutant H510A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 25.5
-
 ADP isoform PPK2, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 29
-
 ATP mutant H510Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 31
-
 ATP mutant H480A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 32.6
-
 ATP mutant H480Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 34.7
-
 ATP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 49
-
 ADP mutant H510A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 57
-
 GDP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 998
-
 ADP mutant Y524A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 3529
-
 ADP mutant H510Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 4690
-
 ADP mutant H480A of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 5222
-
 ADP mutant H480Q of isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.1 5743
-
 ADP isoform PPK1, pH and temperature not specified in the publication Mycobacterium tuberculosis