Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Riedel, T.J.; Johnson, L.C.; Knight, J.; Hantgan, R.R.; Holmes, R.P.; Lowther, W.T.
    Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria (2011), PLoS ONE, 6, e26021.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.16 expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.16 to 1.97 A resolution, crystal structure of enzyme bound to pyruvate. Modeling of the 4-hydroxy-2-oxoglutarate-Schiff base intermediate and kinetic analyses of site-directed mutants support the importance of Lys196 as the nucleophile, Tyr168 and Ser77 as components of a proton relay, and Asn78 and Ser198 as unique residues that facilitate substrate binding Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.16 K196A complete loss of activity Homo sapiens
4.1.3.16 N78A 20% of wild-type activity Homo sapiens
4.1.3.16 N78Q 4% of wild-type activity Homo sapiens
4.1.3.16 N78T 45% of wild-type activity Homo sapiens
4.1.3.16 S198A 9% of wild-type activity Homo sapiens
4.1.3.16 S198T 18% of wild-type activity Homo sapiens
4.1.3.16 S77A 5% of wild-type activity Homo sapiens
4.1.3.16 S77T 2% of wild-type activity Homo sapiens
4.1.3.16 S77V 2% of wild-type activity Homo sapiens
4.1.3.16 Y140F 122% of wild-type activity Homo sapiens
4.1.3.16 Y168F complete loss of activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.16 0.008
-
 4-hydroxy-2-oxoglutarate mutant Y140F, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.011
-
 4-hydroxy-2-oxoglutarate wild-type, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.016
-
 4-hydroxy-2-oxoglutarate mutant N78T, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.044
-
 4-hydroxy-2-oxoglutarate mutant S198A, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.058
-
 4-hydroxy-2-oxoglutarate mutant S77T, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.066
-
 4-hydroxy-2-oxoglutarate mutant N78A, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.077
-
 4-hydroxy-2-oxoglutarate mutant S198T, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.082
-
 4-hydroxy-2-oxoglutarate mutant S77V, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.084
-
 4-hydroxy-2-oxoglutarate mutant S77A, pH 8.5, 37°C Homo sapiens
4.1.3.16 0.281
-
 4-hydroxy-2-oxoglutarate mutant N78Q, pH 8.5, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.16 35249
-
 and dimer, in equilibrium, 4 * 35249, calculated Homo sapiens
4.1.3.16 35249
-
 and tetramer, in equilibrium, 2 * 35249, calculated Homo sapiens
4.1.3.16 97200
-
 sedimentation equilibrium centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Homo sapiens Q86XE5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.16 4-Hydroxy-2-oxoglutarate
-
 Homo sapiens Pyruvate + glyoxylate
-
 r
4.1.3.16 additional information enzyme performs a retro-aldol cleavage reaction reminiscent of the trimeric 2-keto-3-deoxy-6-phosphogluconate aldolases Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.16 dimer and tetramer, in equilibrium, 2 * 35249, calculated Homo sapiens
4.1.3.16 tetramer and dimer, in equilibrium, 4 * 35249, calculated Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.16 0.8
-
 4-hydroxy-2-oxoglutarate mutant S77T, pH 8.5, 37°C Homo sapiens
4.1.3.16 1.2
-
 4-hydroxy-2-oxoglutarate mutant S77V, pH 8.5, 37°C Homo sapiens
4.1.3.16 2.4
-
 4-hydroxy-2-oxoglutarate mutant S198A, pH 8.5, 37°C Homo sapiens
4.1.3.16 2.5
-
 4-hydroxy-2-oxoglutarate mutant S77A, pH 8.5, 37°C Homo sapiens
4.1.3.16 4
-
 4-hydroxy-2-oxoglutarate mutant N78T, pH 8.5, 37°C Homo sapiens
4.1.3.16 5.2
-
 4-hydroxy-2-oxoglutarate mutant Y140F, pH 8.5, 37°C Homo sapiens
4.1.3.16 6.1
-
 4-hydroxy-2-oxoglutarate wild-type, pH 8.5, 37°C Homo sapiens
4.1.3.16 6.2
-
 4-hydroxy-2-oxoglutarate mutant N78Q, pH 8.5, 37°C Homo sapiens
4.1.3.16 7.6
-
 4-hydroxy-2-oxoglutarate mutant N78A, pH 8.5, 37°C Homo sapiens
4.1.3.16 7.8
-
 4-hydroxy-2-oxoglutarate mutant S198T, pH 8.5, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.3.16 14
-
 4-hydroxy-2-oxoglutarate mutant S77T, pH 8.5, 37°C Homo sapiens
4.1.3.16 15
-
 4-hydroxy-2-oxoglutarate mutant S77V, pH 8.5, 37°C Homo sapiens
4.1.3.16 22
-
 4-hydroxy-2-oxoglutarate mutant N78Q, pH 8.5, 37°C Homo sapiens
4.1.3.16 30
-
 4-hydroxy-2-oxoglutarate mutant S77A, pH 8.5, 37°C Homo sapiens
4.1.3.16 55
-
 4-hydroxy-2-oxoglutarate mutant S198A, pH 8.5, 37°C Homo sapiens
4.1.3.16 101
-
 4-hydroxy-2-oxoglutarate mutant S198T, pH 8.5, 37°C Homo sapiens
4.1.3.16 115
-
 4-hydroxy-2-oxoglutarate mutant N78A, pH 8.5, 37°C Homo sapiens
4.1.3.16 250
-
 4-hydroxy-2-oxoglutarate mutant N78T, pH 8.5, 37°C Homo sapiens
4.1.3.16 555
-
 4-hydroxy-2-oxoglutarate wild-type, pH 8.5, 37°C Homo sapiens
4.1.3.16 650
-
 4-hydroxy-2-oxoglutarate mutant Y140F, pH 8.5, 37°C Homo sapiens