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Literature summary extracted from
- Characterization of cis-prenyltransferases from the rubber producing plant Hevea brasiliensis heterologously expressed in yeast and plant cells (2012), Plant Biotechnol., 29, 411-417.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.5.1.20 | additional information | recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity, which seem to be contained in latex species | Hevea brasiliensis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.5.1.20 | recombinant expression of C-terminally His6-tagged HRT2 in Escherichia coli, expression of C-terminally His6-tagged HRT1 amd HRT2 in Saccharomyces cerevisiae and Arabidopsis thaliana T87 cultured cells, predominantly in the membrane fraction in yeast cells | Hevea brasiliensis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.5.1.20 | cytoplasm | - |
Hevea brasiliensis | 5737 | - |
| 2.5.1.20 | additional information | localization of the enzyme on small rubber particle and lutoid particles, which are considered to be polydispersed lysosomal vacuole | Hevea brasiliensis | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.20 | 41000 | - |
x * 41000, recombinant C-terminally His6-tagged HRT1 or HRT2, SDS-PAGE | Hevea brasiliensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.20 | polycis-polyprenyl diphosphate + isopentenyl diphosphate | Hevea brasiliensis | - |
diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.5.1.20 | Hevea brasiliensis | - |
HRT1 and HRT2 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.5.1.20 | latex | - |
Hevea brasiliensis | - |
| 2.5.1.20 | laticifer | - |
Hevea brasiliensis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.20 | additional information | recombinant HRT2 and HRT1 show distinct cPT activity producing polyisoprenoids with chain-lengths of C80-100, although failing to catalyze the formation of natural rubber. Recombinant HRT2, expressed in Escherichia coli, is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber, but the recombinant HRT2 does not exhibit significant activity independently. The chain lengths of the HRT1/HRT2 products are not altered by the addition of centrifuged latex fractions, and the HRT1/HRT2 expressed in yeast compete with the rubber transferase activity of the latex fraction. Recombinantly produced polyprenyl products are apparently smaller than natural rubber molecular weight range | Hevea brasiliensis | ? | - |
? | |
| 2.5.1.20 | polycis-polyprenyl diphosphate + isopentenyl diphosphate | - |
Hevea brasiliensis | diphosphate + a polycis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.5.1.20 | ? | x * 41000, recombinant C-terminally His6-tagged HRT1 or HRT2, SDS-PAGE | Hevea brasiliensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.5.1.20 | cisprenyltransferase | - |
Hevea brasiliensis |
| 2.5.1.20 | CPT | - |
Hevea brasiliensis |
| 2.5.1.20 | HRT1 | - |
Hevea brasiliensis |
| 2.5.1.20 | HRT2 | - |
Hevea brasiliensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.20 | 30 | - |
assay at | Hevea brasiliensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.20 | 7.5 | - |
assay at | Hevea brasiliensis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.5.1.20 | additional information | latex specific co-factor(s) may be required to enable HRT1/HRT2 rubber transferase activity. Recombinant HRT1/HRT2 require additional cofactors from the eukaryotic cells to produce distinct cPT activity | Hevea brasiliensis |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 2.5.1.20 | Hevea brasiliensis | recombinant expression of HRT2 in Escherichia coli is significantly activated in the presence of a centrifuged latex fraction, resulting in the formation of polyisoprenes corresponding to natural rubber | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.5.1.20 | metabolism | HRT2 is thought to be a key enzyme in the biosynthesis of natural rubber | Hevea brasiliensis |
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