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Literature summary extracted from

  • Arthan, D.; Kittakoop, P.; Esen, A.; Svasti, J.
    Furostanol glycoside 26-O-beta-glucosidase from the leaves of Solanum torvum (2006), Phytochemistry, 67, 27-33.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.186 D-glucono-1,5-lactone 1 mM, 44% inhibition. 10 mM, 91% inhibition Solanum torvum
3.2.1.186 Hg2+ 1 mM, 88% loss of activity Solanum torvum
3.2.1.186 Mg2+ 1 mM, 10% inhibition Solanum torvum
3.2.1.186 Zn2+ 1 mM, 12% inhibition Solanum torvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.186 0.063
-
 torvoside A pH 5.0, 37°C Solanum torvum
3.2.1.186 0.068
-
 torvoside H pH 5.0, 37°C Solanum torvum
3.2.1.186 0.78
-
 4-methylumbelliferyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
3.2.1.186 1.03
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 37°C Solanum torvum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.186 80700
-
 1 * 80700, SDS-PAGE Solanum torvum
3.2.1.186 87000
-
 gel filtration Solanum torvum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.186 Solanum torvum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.186 glycoprotein
-
 Solanum torvum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.186
-
 Solanum torvum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.186 leaf young leaves Solanum torvum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.186 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Solanum torvum 4-methylumbelliferol + D-glucopyranose
-
 ?
3.2.1.186 4-nitrophenyl beta-D-glucoside + H2O
-
 Solanum torvum 4-nitrophenol + D-glucose
-
 ?
3.2.1.186 dalcochinin 8'-beta-D-glucoside + H2O the natural substrate of Thai rosewood beta-glucosidase. 24% of the activity with torvoside A Solanum torvum ?
-
 ?
3.2.1.186 additional information no activity with 4-nitrophenyl beta-D-fucoside, 4-nitrophenyl beta-L-fucoside, 4-nitrophenyl beta-D-xyloside, 4-nitrophenyl beta-D-galactoside, 4-nitrophenyl beta-D-N-acetylglucosaminide, 4-nitrophenyl beta-D-mannoside, amygdalin, laminarin, prunasin, glucosinolate (sinigrin), aromatic glucosides (arbutin, salicin), indoxyl-beta-D-glucoside, methyl-beta-D-glucoside, cellobiose, cellotriose, cellotetraose, laminaribiose, laminaritriose, laminaritetraose and gentiobiose Solanum torvum ?
-
 ?
3.2.1.186 torvoside A
-
 Solanum torvum 26-deglucotorvoside A + D-glucose
-
 ?
3.2.1.186 torvoside H
-
 Solanum torvum 26-deglucotorvoside H + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.186 monomer 1 * 80700, SDS-PAGE Solanum torvum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.186 torvosidase
-
 Solanum torvum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.186 37
-
 assay at Solanum torvum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.186 7.7
-
 torvoside H pH 5.0, 37°C Solanum torvum
3.2.1.186 8.3
-
 4-methylumbelliferyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
3.2.1.186 8.6
-
 torvoside A pH 5.0, 37°C Solanum torvum
3.2.1.186 9.1
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 37°C Solanum torvum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.186 5
-
 reaction with p-nitrophenyl beta-D-glucoside or torvoside H Solanum torvum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.186 Solanum torvum isoelectric focusing
-
 8.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.186 8.8
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
3.2.1.186 10.7
-
 4-methylumbelliferyl beta-D-glucoside pH 5.0, 37°C Solanum torvum
3.2.1.186 113
-
 torvoside H pH 5.0, 37°C Solanum torvum
3.2.1.186 136.5
-
 torvoside A pH 5.0, 37°C Solanum torvum