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Literature summary extracted from

  • von Heimendahl, C.B.; Schaefer, K.M.; Eklund, P.; Sjoeholm, R.; Schmidt, T.J.; Fuss, E.
    Pinoresinol-lariciresinol reductases with different stereospecificity from Linum album and Linum usitatissimum (2005), Phytochemistry, 66, 1254-1263.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.23.1.1 expressed in Escherichia coli BL21(DE3)pLysS cells Linum album
1.23.1.2 expressed in Escherichia coli BL21(DE3)pLysS cells Linum album
1.23.1.3 expressed in Escherichia coli BL21(DE3)pLysS cells Linum usitatissimum
1.23.1.4 expressed in Escherichia coli BL21(DE3)pLysS cells Linum usitatissimum

Organism

EC Number Organism UniProt Comment Textmining
1.23.1.1 Linum album Q4R0I0
-
-
1.23.1.2 Linum album Q4R0I0
-
-
1.23.1.3 Linum usitatissimum Q4R0H9
-
-
1.23.1.4 Linum usitatissimum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.23.1.1 Ni-NTA column chromatography Linum album
1.23.1.2 Ni-NTA column chromatography Linum album
1.23.1.3 Ni-NTA column chromatography Linum usitatissimum
1.23.1.4 Ni-NTA column chromatography Linum usitatissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.23.1.1 (+)-pinoresinol + NADPH + H+
-
 Linum album (+)-lariciresinol + NADP+
-
 ?
1.23.1.2 (+)-lariciresinol + NADPH + H+
-
 Linum album (-)-secoisolariciresinol + NADP+
-
 ?
1.23.1.3 (-)-pinoresinol + NADPH + H+
-
 Linum usitatissimum (-)-lariciresinol + NADP+
-
 ?
1.23.1.4 (-)-lariciresinol + NADPH + H+
-
 Linum usitatissimum (+)-secoisolariciresinol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.23.1.1 pinoresinol-lariciresinol reductase
-
 Linum album
1.23.1.1 PLR-La1
-
 Linum album
1.23.1.2 pinoresinol-lariciresinol reductase
-
 Linum album
1.23.1.2 PLR-La1
-
 Linum album
1.23.1.3 pinoresinol-lariciresinol reductase
-
 Linum usitatissimum
1.23.1.3 PLR-Lu1
-
 Linum usitatissimum
1.23.1.4 pinoresinol-lariciresinol reductase
-
 Linum usitatissimum
1.23.1.4 PLR-Lu1
-
 Linum usitatissimum

Cofactor

EC Number Cofactor Comment Organism Structure
1.23.1.1 NADPH
-
 Linum album
1.23.1.3 NADPH
-
 Linum usitatissimum
1.23.1.4 NADPH
-
 Linum usitatissimum