BRENDA - Enzyme Database

Arginine kinase of the sheep blowfly Lucilia cuprina: Gene identification and characterization of the native and recombinant enzyme

Ilg, T.; Werr, M.; Pestic. Biochem. Physiol. 102, 115-123 (2012)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.3.3
expressed in Escherichia coli M15 cells
Lucilia cuprina
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.594
-
L-Arg
native enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
0.603
-
L-Arg
recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
1.166
-
Nomega-phospho-L-Arg
native enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
1.432
-
Nomega-phospho-L-Arg
recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
2.796
-
L-arginine ethyl ester
recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.3.3
soluble
-
Lucilia cuprina
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
x * 40000, SDS-PAGE
Lucilia cuprina
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Lucilia cuprina
H9BZ68
Australian sheep blowfly
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
Ni2+-NTA agarose column chromatography
Lucilia cuprina
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.3.3
abdomen
-
Lucilia cuprina
-
2.7.3.3
head
-
Lucilia cuprina
-
2.7.3.3
thorax
the enzyme is more abundant in the thorax, where it represents up to 2% of the total soluble protein, compared to head or abdomen
Lucilia cuprina
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ADP + Nomega-phospho-L-Arg
-
723344
Lucilia cuprina
ATP + L-Arg
-
-
-
r
2.7.3.3
ATP + L-Arg
the enzyme is stereospecific for the L-form over the D-form of its specific substrate arginine
723344
Lucilia cuprina
ADP + Nomega-phospho-L-Arg
-
-
-
r
2.7.3.3
ATP + L-arginine ethyl ester
6% activity compared to L-Arg
723344
Lucilia cuprina
ADP + Nomega-phospho-L-Arg ethyl ester
-
-
-
?
2.7.3.3
ATP + L-canavanine
about 16% activity compared to L-Arg
723344
Lucilia cuprina
?
-
-
-
?
2.7.3.3
ATP + L-homoarginine
7% activity compared to L-Arg
723344
Lucilia cuprina
ADP + Nomega-phospho-L-homoarginine
-
-
-
?
2.7.3.3
additional information
no activity with D-arginine, N-acetyl-L-arginine, agmatine, and creatine
723344
Lucilia cuprina
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
x * 40000, SDS-PAGE
Lucilia cuprina
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
expressed in Escherichia coli M15 cells
Lucilia cuprina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.594
-
L-Arg
native enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
0.603
-
L-Arg
recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
1.166
-
Nomega-phospho-L-Arg
native enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
1.432
-
Nomega-phospho-L-Arg
recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
2.7.3.3
2.796
-
L-arginine ethyl ester
recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C
Lucilia cuprina
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.3.3
soluble
-
Lucilia cuprina
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
x * 40000, SDS-PAGE
Lucilia cuprina
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
Ni2+-NTA agarose column chromatography
Lucilia cuprina
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.3.3
abdomen
-
Lucilia cuprina
-
2.7.3.3
head
-
Lucilia cuprina
-
2.7.3.3
thorax
the enzyme is more abundant in the thorax, where it represents up to 2% of the total soluble protein, compared to head or abdomen
Lucilia cuprina
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ADP + Nomega-phospho-L-Arg
-
723344
Lucilia cuprina
ATP + L-Arg
-
-
-
r
2.7.3.3
ATP + L-Arg
the enzyme is stereospecific for the L-form over the D-form of its specific substrate arginine
723344
Lucilia cuprina
ADP + Nomega-phospho-L-Arg
-
-
-
r
2.7.3.3
ATP + L-arginine ethyl ester
6% activity compared to L-Arg
723344
Lucilia cuprina
ADP + Nomega-phospho-L-Arg ethyl ester
-
-
-
?
2.7.3.3
ATP + L-canavanine
about 16% activity compared to L-Arg
723344
Lucilia cuprina
?
-
-
-
?
2.7.3.3
ATP + L-homoarginine
7% activity compared to L-Arg
723344
Lucilia cuprina
ADP + Nomega-phospho-L-homoarginine
-
-
-
?
2.7.3.3
additional information
no activity with D-arginine, N-acetyl-L-arginine, agmatine, and creatine
723344
Lucilia cuprina
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
x * 40000, SDS-PAGE
Lucilia cuprina