Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ilg, T.; Werr, M.
    Arginine kinase of the sheep blowfly Lucilia cuprina: Gene identification and characterization of the native and recombinant enzyme (2012), Pestic. Biochem. Physiol., 102, 115-123.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 expressed in Escherichia coli M15 cells Lucilia cuprina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.594
-
 L-Arg native enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C Lucilia cuprina
2.7.3.3 0.603
-
 L-Arg recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C Lucilia cuprina
2.7.3.3 1.166
-
 Nomega-phospho-L-Arg native enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C Lucilia cuprina
2.7.3.3 1.432
-
 Nomega-phospho-L-Arg recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C Lucilia cuprina
2.7.3.3 2.796
-
 L-arginine ethyl ester recombinant enzyme, in 50 mM Tris–HCl pH 7.5, at 22°C Lucilia cuprina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.3 soluble
-
 Lucilia cuprina
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 40000
-
 x * 40000, SDS-PAGE Lucilia cuprina

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Lucilia cuprina H9BZ68 Australian sheep blowfly
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3 Ni2+-NTA agarose column chromatography Lucilia cuprina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.3 abdomen
-
 Lucilia cuprina
-
2.7.3.3 head
-
 Lucilia cuprina
-
2.7.3.3 thorax the enzyme is more abundant in the thorax, where it represents up to 2% of the total soluble protein, compared to head or abdomen Lucilia cuprina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ADP + Nomega-phospho-L-Arg
-
 Lucilia cuprina ATP + L-Arg
-
 r
2.7.3.3 ATP + L-Arg the enzyme is stereospecific for the L-form over the D-form of its specific substrate arginine Lucilia cuprina ADP + Nomega-phospho-L-Arg
-
 r
2.7.3.3 ATP + L-arginine ethyl ester 6% activity compared to L-Arg Lucilia cuprina ADP + Nomega-phospho-L-Arg ethyl ester
-
 ?
2.7.3.3 ATP + L-canavanine about 16% activity compared to L-Arg Lucilia cuprina ?
-
 ?
2.7.3.3 ATP + L-homoarginine 7% activity compared to L-Arg Lucilia cuprina ADP + Nomega-phospho-L-homoarginine
-
 ?
2.7.3.3 additional information no activity with D-arginine, N-acetyl-L-arginine, agmatine, and creatine Lucilia cuprina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.3 ? x * 40000, SDS-PAGE Lucilia cuprina

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 ATP:L-arginine omega-N-phosphotransferase
-
 Lucilia cuprina