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Literature summary extracted from

  • Berger, K.L.; Kelly, S.M.; Jordan, T.X.; Tartell, M.A.; Randall, G.
    Hepatitis C virus stimulates the phosphatidylinositol 4-kinase III alpha-dependent phosphatidylinositol 4-phosphate production that is essential for its replication (2011), J. Virol., 85, 8870-8883.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.67 NS5A stimulates phosphatidylinositol 4-phosphate production in vivo and enhances PI4KA kinase activity in vitro, but not other viral proteins from hepatitis C virus, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.67 isozyme PI4KA, DNA and amino acid sequence determination and analysis, expression of GFP-tagged wild-type and mutant enzymes in HEK-293T cells and in U2OS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.67 K1792L site-direcetd mutagenesis, inactive isozyme PI4KA Homo sapiens
2.7.1.67 additional information isozyme PI4KA depletion by siRNA expression in Huh-7.5 cells, transcomplementation, complementation with the siRNA-resistant PI4KA enhances infectious HCV production 110fold compared to the vector alone, overview. In cell lines expressing the HCV polyprotein, PI4KA siRNAs alter the localization of the HCV NS5A protein, while membranous webs are difficult to detect in HCV-infected cells pretreated with PI4KA siRNAs Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 cytoplasm
-
 Homo sapiens 5737
-
2.7.1.67 endoplasmic reticulum
-
 Homo sapiens 5783
-
2.7.1.67 additional information phosphatidylinositol 4-phosphate localization has a distinct pattern in cells infected with hepatitis C virus (extensive cytoplasmic distribution) compared to uninfected cells Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.67 Huh-7.5 cell
-
 Homo sapiens
-
2.7.1.67 U2-OS cell cell lines with inducible expression of the HCV polyprotein or individual viral proteins Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.67 phosphatidylinositol 4-kinase III alpha
-
 Homo sapiens
2.7.1.67 PI4KA
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.67 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.67 malfunction siRNAs that reduce PI4KA accumulation appear to perturb membranous web formation. Cell viral polyprotein expression results in enhanced cytoplasmic phosphatidylinositol 4-phosphate production. Increased phosphatidylinositol 4-phosphate accumulation following hepatitis C virus protein expression is precluded by silencing the expression of isozyme PI4KA, but not the related isozyme PI4KB. Silencing PI4KA also results in aberrant agglomeration of viral replicase proteins, including NS5A, NS5B, and NS3. NS5A alone, but not other viral proteins, stimulates phosphatidylinositol 4-phosphate production in vivo and enhanced PI4KA kinase activity in vitro Homo sapiens
2.7.1.67 additional information hepatitis C virus stimulates the phosphatidylinositol 4-kinase III alpha-dependent phosphatidylinositol 4-phosphate production that is essential for its replication, overview. PI4KA is also implicated in HCV replication complex formation Homo sapiens