BRENDA - Enzyme Database show

Novel PET probes specific for deoxycytidine kinase

Shu, C.J.; Campbell, D.O.; Lee, J.T.; Tran, A.Q.; Wengrod, J.C.; Witte, O.N.; Phelps, M.E.; Satyamurthy, N.; Czernin, J.; Radu, C.G.; J. Nucl. Med. 51, 1092-1098 (2010)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.7.1.74
pharmacology
the enzyme is a critical determinant of therapeutic activity for several nucleoside analogue prodrugs
Homo sapiens
2.7.1.74
pharmacology
the enzyme is a critical determinant of therapeutic activity for several nucleoside analogue prodrugs
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00061
-
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.00076
-
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.00102
-
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.00654
-
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
2.7.1.74
cytosol
-
Homo sapiens
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
required
Mus musculus
2.7.1.74
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + deoxyadenosine
Mus musculus
-
ADP + dAMP
-
-
?
2.7.1.74
ATP + deoxyadenosine
Homo sapiens
-
ADP + dAMP
-
-
?
2.7.1.74
ATP + deoxyadenosine
Mus musculus C57/BL6J
-
ADP + dAMP
-
-
?
2.7.1.74
ATP + deoxycytidine
Mus musculus
-
ADP + dCMP
-
-
?
2.7.1.74
ATP + deoxycytidine
Homo sapiens
-
ADP + dCMP
-
-
?
2.7.1.74
ATP + deoxycytidine
Mus musculus C57/BL6J
-
ADP + dCMP
-
-
?
2.7.1.74
ATP + deoxyguanosine
Mus musculus
-
ADP + dGMP
-
-
?
2.7.1.74
ATP + deoxyguanosine
Homo sapiens
-
ADP + dGMP
-
-
?
2.7.1.74
ATP + deoxyguanosine
Mus musculus C57/BL6J
-
ADP + dGMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
2.7.1.74
Mus musculus
-
-
-
2.7.1.74
Mus musculus C57/BL6J
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
additional information
dCK-negative L1210-10K tumors
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine monophosphate
-
-
-
?
2.7.1.74
ATP + clofarabine
-
723029
Mus musculus
ADP + clofarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + clofarabine
-
723029
Homo sapiens
ADP + clofarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + clofarabine
-
723029
Mus musculus C57/BL6J
ADP + clofarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + deoxyadenosine
-
723029
Mus musculus
ADP + dAMP
-
-
-
?
2.7.1.74
ATP + deoxyadenosine
-
723029
Homo sapiens
ADP + dAMP
-
-
-
?
2.7.1.74
ATP + deoxyadenosine
-
723029
Mus musculus C57/BL6J
ADP + dAMP
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723029
Mus musculus
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723029
Homo sapiens
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723029
Mus musculus C57/BL6J
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + deoxyguanosine
-
723029
Mus musculus
ADP + dGMP
-
-
-
?
2.7.1.74
ATP + deoxyguanosine
-
723029
Homo sapiens
ADP + dGMP
-
-
-
?
2.7.1.74
ATP + deoxyguanosine
-
723029
Mus musculus C57/BL6J
ADP + dGMP
-
-
-
?
2.7.1.74
additional information
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
723029
Mus musculus
?
-
-
-
-
2.7.1.74
additional information
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
723029
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
723029
Mus musculus C57/BL6J
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
2.7.1.74
37
-
assay at
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.6
-
assay at
Homo sapiens
2.7.1.74
7.6
-
assay at
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Mus musculus
2.7.1.74
ATP
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.1.74
pharmacology
the enzyme is a critical determinant of therapeutic activity for several nucleoside analogue prodrugs
Homo sapiens
2.7.1.74
pharmacology
the enzyme is a critical determinant of therapeutic activity for several nucleoside analogue prodrugs
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.74
ATP
-
Mus musculus
2.7.1.74
ATP
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00061
-
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.00076
-
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.00102
-
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
2.7.1.74
0.00654
-
1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
pH 7.6, 37°C, recombinant enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.74
cytosol
-
Mus musculus
5829
-
2.7.1.74
cytosol
-
Homo sapiens
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.74
Mg2+
required
Mus musculus
2.7.1.74
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
ATP + deoxyadenosine
Mus musculus
-
ADP + dAMP
-
-
?
2.7.1.74
ATP + deoxyadenosine
Homo sapiens
-
ADP + dAMP
-
-
?
2.7.1.74
ATP + deoxyadenosine
Mus musculus C57/BL6J
-
ADP + dAMP
-
-
?
2.7.1.74
ATP + deoxycytidine
Mus musculus
-
ADP + dCMP
-
-
?
2.7.1.74
ATP + deoxycytidine
Homo sapiens
-
ADP + dCMP
-
-
?
2.7.1.74
ATP + deoxycytidine
Mus musculus C57/BL6J
-
ADP + dCMP
-
-
?
2.7.1.74
ATP + deoxyguanosine
Mus musculus
-
ADP + dGMP
-
-
?
2.7.1.74
ATP + deoxyguanosine
Homo sapiens
-
ADP + dGMP
-
-
?
2.7.1.74
ATP + deoxyguanosine
Mus musculus C57/BL6J
-
ADP + dGMP
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
additional information
dCK-negative L1210-10K tumors
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-bromocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-chlorocytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
-
723029
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine monophosphate
-
-
-
?
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine
-
723029
Homo sapiens
ADP + 1-(2'-deoxy-2'-fluoro-beta-L-arabinofuranosyl)-5-methylcytosine monophosphate
-
-
-
?
2.7.1.74
ATP + clofarabine
-
723029
Mus musculus
ADP + clofarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + clofarabine
-
723029
Homo sapiens
ADP + clofarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + clofarabine
-
723029
Mus musculus C57/BL6J
ADP + clofarabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + deoxyadenosine
-
723029
Mus musculus
ADP + dAMP
-
-
-
?
2.7.1.74
ATP + deoxyadenosine
-
723029
Homo sapiens
ADP + dAMP
-
-
-
?
2.7.1.74
ATP + deoxyadenosine
-
723029
Mus musculus C57/BL6J
ADP + dAMP
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723029
Mus musculus
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723029
Homo sapiens
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + deoxycytidine
-
723029
Mus musculus C57/BL6J
ADP + dCMP
-
-
-
?
2.7.1.74
ATP + deoxyguanosine
-
723029
Mus musculus
ADP + dGMP
-
-
-
?
2.7.1.74
ATP + deoxyguanosine
-
723029
Homo sapiens
ADP + dGMP
-
-
-
?
2.7.1.74
ATP + deoxyguanosine
-
723029
Mus musculus C57/BL6J
ADP + dGMP
-
-
-
?
2.7.1.74
additional information
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
723029
Mus musculus
?
-
-
-
-
2.7.1.74
additional information
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
723029
Homo sapiens
?
-
-
-
-
2.7.1.74
additional information
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil is no substrate. But 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine can be converted to 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)uracil by cytidine deaminase
723029
Mus musculus C57/BL6J
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.1.74
37
-
assay at
Homo sapiens
2.7.1.74
37
-
assay at
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.74
7.6
-
assay at
Homo sapiens
2.7.1.74
7.6
-
assay at
Mus musculus
General Information
EC Number
General Information
Commentary
Organism
2.7.1.74
metabolism
deoxycytidine kinase is a rate-limiting enzyme in the deoxyribonucleoside salvage pathway
Homo sapiens
2.7.1.74
metabolism
deoxycytidine kinase is a rate-limiting enzyme in the deoxyribonucleoside salvage pathway
Mus musculus
2.7.1.74
additional information
biodistribution of cytosine derivative substrates in mouse healthy and malignant tissues, overview
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.74
metabolism
deoxycytidine kinase is a rate-limiting enzyme in the deoxyribonucleoside salvage pathway
Homo sapiens
2.7.1.74
metabolism
deoxycytidine kinase is a rate-limiting enzyme in the deoxyribonucleoside salvage pathway
Mus musculus
2.7.1.74
additional information
biodistribution of cytosine derivative substrates in mouse healthy and malignant tissues, overview
Mus musculus