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Literature summary extracted from

  • Wubben, T.J.; Mesecar, A.D.
    Kinetic, thermodynamic, and structural insight into the mechanism of phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis (2010), J. Mol. Biol., 404, 202-219.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.3 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.3 enzyme in complex with pantetheine 4'-phosphate and adenosine-5'-[(alpha,beta)-methyleno]triphosphate, sitting drop vapor diffusion method, using 38% (v/v) polyethylene glycol 200 and 0.1 M HEPES (pH 7.2), at 21°C Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.3 adenosine-5'-[(alpha,beta)-methyleno]triphosphate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.3 0.0067
-
 3'-dephospho-CoA apparent value, at 25°C in 50 mM Tris (pH 8.0) Mycobacterium tuberculosis
2.7.7.3 0.0377
-
 pantetheine 4'-phosphate apparent value, at 25°C in 75 mM HEPES (pH 7.0) Mycobacterium tuberculosis
2.7.7.3 0.1157
-
 ATP apparent value, at 25°C in 75 mM HEPES (pH 7.0) Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.3 Mg2+ required for activity Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.3 Mycobacterium tuberculosis P9WPA5
-
-
2.7.7.3 Mycobacterium tuberculosis H37Rv P9WPA5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.3 Ni-NTA agarose column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Mycobacterium tuberculosis diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 ATP + pantetheine 4'-phosphate
-
 Mycobacterium tuberculosis H37Rv diphosphate + 3'-dephospho-CoA
-
 r
2.7.7.3 diphosphate + 3'-dephospho-CoA
-
 Mycobacterium tuberculosis ATP + pantetheine 4'-phosphate
-
 r
2.7.7.3 diphosphate + 3'-dephospho-CoA
-
 Mycobacterium tuberculosis H37Rv ATP + pantetheine 4'-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.3 homohexamer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.3 phosphopantetheine adenylyltransferase
-
 Mycobacterium tuberculosis
2.7.7.3 PPAT
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.3 4.547
-
 ATP at 25°C in 75 mM HEPES (pH 7.0) Mycobacterium tuberculosis
2.7.7.3 4.808
-
 3'-dephospho-CoA at 25°C in 50 mM Tris (pH 8.0) Mycobacterium tuberculosis
2.7.7.3 4.853
-
 pantetheine 4'-phosphate at 25°C in 75 mM HEPES (pH 7.0) Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.3 0.388
-
 adenosine-5'-[(alpha,beta)-methyleno]triphosphate at 25°C in 75 mM HEPES (pH 7.0) Mycobacterium tuberculosis