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Literature summary extracted from

  • Ringpis, G.E.; Stagno, J.; Aphasizhev, R.
    Mechanism of U-insertion RNA editing in trypanosome mitochondria: characterization of RET2 functional domains by mutational analysis (2010), J. Mol. Biol., 399, 696-706.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.52
-
 Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.52 C83F 230% of wild-type activity for transfer to terminal U residue Trypanosoma brucei
2.7.7.52 D97A complete inactivation of uridylyl transfer Trypanosoma brucei
2.7.7.52 K149A mutant retains partial activity Trypanosoma brucei
2.7.7.52 R141A almost complete inactivation of uridylyl transfer Trypanosoma brucei
2.7.7.52 R144A almost complete inactivation of uridylyl transfer Trypanosoma brucei
2.7.7.52 R435A almost complete inactivation of uridylyl transfer Trypanosoma brucei
2.7.7.52 V271R 10-20% of wild-type activity Trypanosoma brucei
2.7.7.52 V271R/C83F 240% of wild-type activity for transfer to terminal U residue Trypanosoma brucei
2.7.7.52 Y319A complete inactivation of uridylyl transfer Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.52 0.0005
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0006
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0025
-
 UTP wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0026
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0028
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.003
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0081
-
 UTP mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0126
-
 UTP wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0209
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 UTP + RNAn invariant arginine residues 144 and 435 positioned in the vicinity of the UTP-binding site are critical for isoform RET2 activity on single-stranded and double-stranded RNAs, as well as function in vivo. Recognition of a double-stranded RNA, which resembles a guide RNA/mRNA duplex, is further facilitated by multipoint contacts across the RET2-specific middle domain Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn containing a terminal G residue substrate dsRNA Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn containing a terminal U residue substrate dsRNA Trypanosoma brucei diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.52 RET2
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.52 0.00002
-
 UTP mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.00003
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.00005
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.00012
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0003
-
 UTP wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0012
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0015
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0018
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.003
-
 UTP wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.52 0.0053
-
 UTP mutant K149A, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0058
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0097
-
 UTP mutant V271R, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0567
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.0867
-
 UTP mutant C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.117
-
 UTP wild-type, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.21
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal G residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.25
-
 UTP wild-type, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei
2.7.7.52 0.6
-
 UTP mutant V271R/C83F, cosubstrate RNAn containing a terminal U residue, pH 8.0, temperature not specified in the publication Trypanosoma brucei