BRENDA - Enzyme Database show

Tungsten-dependent formaldehyde ferredoxin oxidoreductase: reaction mechanism from quantum chemical calculations

Liao, R.Z.; Yu, J.G.; Himo, F.; J. Inorg. Biochem. 105, 927-936 (2011)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.B2
Tungsten
tungsten-dependent enzyme. A mechanism is proposed in which the formaldehyde substrate binds directly to the tungsten ion. W(VI)=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion
Pyrococcus furiosus
1.2.7.5
Tungsten
dependent on
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.5
formaldehyde + H2O + oxidized ferredoxin
Pyrococcus furiosus
-
formate + H+ + reduced ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.B2
Pyrococcus furiosus
-
-
-
1.2.7.5
Pyrococcus furiosus
Q8U1K3
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.2.7.B2
formaldehyde + H2O + 2 oxidized ferredoxin = formate + 2 H+ + 2 reduced ferredoxin
a mechanism is proposed in which the formaldehyde substrate binds directly to the tungsten ion. W(VI)=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.5
formaldehyde + H2O + oxidized ferredoxin
-
722893
Pyrococcus furiosus
formate + H+ + reduced ferredoxin
-
-
-
?
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.B2
Tungsten
tungsten-dependent enzyme. A mechanism is proposed in which the formaldehyde substrate binds directly to the tungsten ion. W(VI)=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion
Pyrococcus furiosus
1.2.7.5
Tungsten
dependent on
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.7.5
formaldehyde + H2O + oxidized ferredoxin
Pyrococcus furiosus
-
formate + H+ + reduced ferredoxin
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.5
formaldehyde + H2O + oxidized ferredoxin
-
722893
Pyrococcus furiosus
formate + H+ + reduced ferredoxin
-
-
-
?