EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.292 | adenine | 0.25 mM, 8% inhibition | Streptomyces sp. | |
2.1.1.292 | additional information | homocysteine, adenosine, epsilon-rhodomycinone, daunomycin, and daunomycinone show little or no inhibitory activity | Streptomyces sp. | |
2.1.1.292 | S-adenosyl-L-homocysteine | 0.025 mM, 87% inhibition | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.292 | 0.0005 | - |
carminomycin | pH 8.0, 37°C | Streptomyces sp. | |
2.1.1.292 | 0.001 | - |
13-dihydrocarminomycin | pH 8.0, 37°C | Streptomyces sp. | |
2.1.1.292 | 0.025 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Streptomyces sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.292 | additional information | no divalent cation requirement can be demonstrated for the partially purified enzyme | Streptomyces sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 41000 | - |
4 * 41000, SDS-PAGE | Streptomyces sp. |
2.1.1.292 | 166000 | - |
gel filtration | Streptomyces sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin | Streptomyces sp. | terminal enzyme in the daunomycin biosynthesis pathway | S-adenosyl-L-homocysteine + daunorubicinol | - |
? | |
2.1.1.292 | S-adenosyl-L-methionine + carminomycin | Streptomyces sp. | - |
S-adenosyl-L-homocysteine + daunorubicin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.292 | Streptomyces sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.292 | partial | Streptomyces sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.292 | mycelium | - |
Streptomyces sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 0.0194 | - |
pH 8.0, 37°C | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.292 | additional information | the purified enzyme does not catalyse methylation of the aglycones carminomycinone or 13-dihydrocarminomycinone | Streptomyces sp. | ? | - |
? | |
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin | terminal enzyme in the daunomycin biosynthesis pathway | Streptomyces sp. | S-adenosyl-L-homocysteine + daunorubicinol | - |
? | |
2.1.1.292 | S-adenosyl-L-methionine + 13-dihydrocarminomycin | the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin | Streptomyces sp. | S-adenosyl-L-homocysteine + daunorubicinol | - |
? | |
2.1.1.292 | S-adenosyl-L-methionine + carminomycin | - |
Streptomyces sp. | S-adenosyl-L-homocysteine + daunorubicin | - |
? | |
2.1.1.292 | S-adenosyl-L-methionine + carminomycin | the enzyme shows a slightly higher affinity for carminomycin than for 13-dihydrocarminomycin | Streptomyces sp. | S-adenosyl-L-homocysteine + daunorubicin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.292 | tetramer | 4 * 41000, SDS-PAGE | Streptomyces sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.292 | DnrK | - |
Streptomyces sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 37 | - |
- |
Streptomyces sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 30 | 45 | 30°C: about 90% of maximal activity, 45°C: about 90% of maximal activity | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 8 | - |
- |
Streptomyces sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 7.5 | 8 | pH 7.5: 40% of maximal activity, pH 8.0: optimum. No pH values above pH 8.0 are evaluated due to the inherent instability of S-adenosyl-L-methionine at alkaline pH | Streptomyces sp. |