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Literature summary extracted from

  • Lasseur, B.; Lothier, J.; Wiemken, A.; Van Laere, A.; Morvan-Bertrand, A.; Ende, W.V.; Prudhomme, M.P.
    Towards a better understanding of the generation of fructan structure diversity in plants: molecular and functional characterization of a sucrose:fructan 6-fructosyltransferase (6-SFT) cDNA from perennial ryegrass (Lolium perenne) (2011), J. Exp. Bot., 62, 1871-1885.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.243 FFT phylogenetic tree Lolium perenne

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.243 additional information Lolium perenne Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose ?
-
 ?
2.4.1.243 additional information Lolium perenne Bravo Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.243 Lolium perenne Q8S337
-
-
2.4.1.243 Lolium perenne Bravo Q8S337
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.243 leaf elongating leaves, basal segment Lolium perenne
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.243 1-kestose + sucrose
-
 Lolium perenne 1,1-kestotetraose + 1,6-kestotetraose neither this fructan nor the subsequent mixed series is found in vivo in the tissues of Lolium perenne ?
2.4.1.243 1-kestose + sucrose
-
 Lolium perenne Bravo 1,1-kestotetraose + 1,6-kestotetraose neither this fructan nor the subsequent mixed series is found in vivo in the tissues of Lolium perenne ?
2.4.1.243 1-kestose + sucrose
-
 Lolium perenne 6G,6-kestotetraose
-
 ?
2.4.1.243 1-kestose + sucrose
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 Lolium perenne Bravo 6G,6-kestotetraose
-
 ?
2.4.1.243 additional information Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose Lolium perenne ?
-
 ?
2.4.1.243 additional information both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates Lolium perenne ?
-
 ?
2.4.1.243 additional information Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose Lolium perenne Bravo ?
-
 ?
2.4.1.243 additional information both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates Lolium perenne Bravo ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.243 6G-FFT
-
 Lolium perenne
2.4.1.243 fructan:fructan 6G-fructosyltransferase
-
 Lolium perenne

General Information

EC Number General Information Comment Organism
2.4.1.243 metabolism the enzyme is required for fructan biosynthesis. In the second step, a 6G-FFT produces 6G-kestotriose, using 1-kestotriose as fructosyl donor and sucrose as fructosyl acceptor. In further steps, higher polymeric fructans are synthesized by a 1-FFT activity through beta(2-1)-linked chain elongation or by a 6-FT activity through beta(2-6)-linked chain elongation.The 6G-FFT from Lolium perenne also has 1-FFT activity Lolium perenne