Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Floyd, N.; Liebster, M.; Turner, N.
    A simple strategy for obtaining both enantiomers from an aldolase reaction: Preparation of L- and D-4-hydroxy-2-ketoglutarate (1992), J. Chem. Soc. Perkin Trans. I, 1992, 1085-1086.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.1.3.42 synthesis preparation of (4S)-4-hydroxy-2-oxoglutarate at more than 95% enantiomeric excess by stereospecific synthesis from glyoxylate and pyruvate. Preparation of (4R)-4-hydroxy-2-oxoglutarate at 60% enantiomeric excess by selective cleavage of the (4S)-isomer of racemic 4-hydroxy-2-oxoglutarate leaving the (4R)-isomer in solution Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.42 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.42 D,L-4-hydroxy-2-oxoglutarate
-
 Escherichia coli pyruvate + glyoxylate + D-4-hydroxy-2-oxoglutarate preferential cleavage of the L-isomer of racemic 4-hydroxy-2-oxoglutarate leaving the D-isomer in solution r
4.1.3.42 additional information enzyme shows high specificity for both glyoxylate and pyruvate Escherichia coli ?
-
 ?
4.1.3.42 pyruvate + glyoxylate
-
 Escherichia coli (4S)-4-hydroxy-2-oxoglutarate i.e. L-4-hydroxy-2-oxoglutarate. Condensation of pyruvate and glyoxylate carried out at a relatively high concentration leads to a quantitative conversion of pyruvate and glyoxylate to (4S)-4-hydroxy-2-oxoglutarate r