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Literature summary extracted from

  • Dohi, K.; Isoyama-Tanaka, J.; Tokuda, T.; Fujiyama, K.
    Recombinant expression and characterization of N-acetylglucosaminyltransferase I derived from Nicotiana tabacum (2010), J. Biosci. Bioeng., 109, 388-391.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.101 the enzyme fused to maltose-binding protein is expressed in Escherichia coli Rosetta-gami B(DE3) cells Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.101 0.5
-
 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside recombinant enzyme, pH not specified in the publication, at 25°C Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.101 Golgi apparatus
-
 Nicotiana tabacum 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.101 Mn2+ maximal activity in the presence of 2 mM Mn2+ Nicotiana tabacum
2.4.1.101 additional information not stimulated by Mg2+, Co2+, Ca2+, Zn2+ and C2+ Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Nicotiana tabacum Q9ST97
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.101 amylose resin column chromatography, gel filtration Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside 3.9% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside Nicotiana tabacum UDP + GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside 0.5% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside Nicotiana tabacum UDP + Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside 0.7% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside Nicotiana tabacum UDP + GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside 100% activity Nicotiana tabacum UDP + Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside 6.9% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside Nicotiana tabacum UDP + Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside 6.2% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside Nicotiana tabacum UDP + Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.101 GnT I
-
 Nicotiana tabacum
2.4.1.101 N-acetylglucosaminyltransferase I
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.101 25
-
-
 Nicotiana tabacum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.101
-
 45 the activity decreases with increasing reaction temperature and is completely abolished at temperatures higher than 45°C. On the other hand, substantial GnTI activity is detected at low temperatures, even at 0°C (approximately 50% of the maximum) Nicotiana tabacum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.101 25 45 the activity is stable after a 2 h incubation at a temperature lower than 25°C, but it significantly decreases after incubation at 35°C and is completely abolished at temperatures higher than 45°C Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.101 6.3
-
-
 Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.101 5 8.5 activity decreases to less than 15% of the maximum at pH values lower than 5.0 and higher than 8.5 Nicotiana tabacum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.101 5 10 the enzymatic activity is maintained after a 90-min incubation at pH values 6.0-9.5, but it is mostly abolished at pH values 5.0 and 10.0 Nicotiana tabacum