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Literature summary extracted from

  • Shniffer, A.; Visschedyk, D.D.; Ravulapalli, R.; Suarez, G.; Turgeon, Z.J.; Petrie, A.A.; Chopra, A.K.; Merrill, A.R.
    Characterization of an actin-targeting ADP-ribosyltransferase from Aeromonas hydrophila (2012), J. Biol. Chem., 287, 37030-37041.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.30
-
 Aeromonas hydrophila

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.30 E213A kcat highly decreased compared to wild-type, Km similar to wild-type Aeromonas hydrophila
2.4.2.30 E215A kcat highly decreased compared to wild-type, Km (ADP-D-ribosyl)n-actin similar to wild-type, Km (NAD+) increased compared to wild-type Aeromonas hydrophila
2.4.2.30 N86A kcat moderately decreased compared to wild-type, Km decreased compared to wild-type Aeromonas hydrophila
2.4.2.30 S178A kcat decreased compared to wild-type, Km increased compared to wild-type Aeromonas hydrophila
2.4.2.30 Y77A kcat decreased compared to wild-type, Km increased compared to wild-type Aeromonas hydrophila
2.4.2.30 Y82A kcat decreased compared to wild-type, Km increased compared to wild-type Aeromonas hydrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.30 2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol
-
 Aeromonas hydrophila
2.4.2.30 N-(acridin-9-yl)-4-nitrobenzamide
-
 Aeromonas hydrophila
2.4.2.30 N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide
-
 Aeromonas hydrophila
2.4.2.30 suramin
-
 Aeromonas hydrophila
2.4.2.30 V8 protease
-
 Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.30 0.004
-
 NAD+ mutant N86A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.005
-
 NAD+ mutant E213A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.006
-
 NAD+ wild-type, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.008
-
 NAD+ mutant Y77A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.011
-
 (ADP-D-ribosyl)n-actin mutant N86A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.012
-
 NAD+ mutant Y82A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.014
-
 NAD+ mutant E215A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.018
-
 NAD+ mutant S178A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.02
-
 (ADP-D-ribosyl)n-actin mutant E213A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.023
-
 (ADP-D-ribosyl)n-actin mutant E215A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.024
-
 (ADP-D-ribosyl)n-actin wild-type, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.033
-
 (ADP-D-ribosyl)n-actin mutant S178A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.035
-
 (ADP-D-ribosyl)n-actin mutant Y82A, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.037
-
 (ADP-D-ribosyl)n-actin mutant Y77A, 25°C, pH not specified in the publication Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Aeromonas hydrophila Q49TP5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-actin VahC is shown to ADP-ribosylate Arg-177 of actin. VahC activity causes depolymerization of actin filaments, which induces caspase-mediated apoptosis in HeLa Tet-Off cells Aeromonas hydrophila nicotinamide + (ADP-D-ribosyl)n+1-actin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.30 mART
-
 Aeromonas hydrophila
2.4.2.30 mono-ADP-ribosyltransferase
-
 Aeromonas hydrophila
2.4.2.30 VahC
-
 Aeromonas hydrophila
2.4.2.30 vsdc
-
 Aeromonas hydrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.30 0.05
-
 NAD+ mutant E215A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.05
-
 (ADP-D-ribosyl)n-actin mutant E215A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.11
-
 NAD+ mutant E213A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.11
-
 (ADP-D-ribosyl)n-actin mutant E213A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.65
-
 NAD+ mutant Y82A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.65
-
 (ADP-D-ribosyl)n-actin mutant Y82A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 1.3
-
 NAD+ mutant Y77A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 1.3
-
 (ADP-D-ribosyl)n-actin mutant Y77A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 1.38
-
 NAD+ mutant S178, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 1.38
-
 (ADP-D-ribosyl)n-actin mutant S178A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 9.35
-
 NAD+ mutant N86A, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 9.35
-
 (ADP-D-ribosyl)n-actin mutant N86A, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 22
-
 NAD+ wild-type, co-substrate: (ADP-D-ribosyl)n-actin, 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 22
-
 (ADP-D-ribosyl)n-actin wild-type, co-substrate: NAD+, 25°C, pH not specified in the publication Aeromonas hydrophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.30 0.001
-
 suramin 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.0041
-
 N-(acridin-9-yl)-4-nitrobenzamide 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.0041
-
 N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide 25°C, pH not specified in the publication Aeromonas hydrophila
2.4.2.30 0.0075
-
 2-(4-[4-[(2,4-dimethoxyphenyl)amino]quinazolin-2-yl]piperazin-1-yl)ethanol 25°C, pH not specified in the publication Aeromonas hydrophila

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.30 0.02
-
 25°C, pH not specified in the publication Aeromonas hydrophila suramin
2.4.2.30 0.0858
-
 25°C, pH not specified in the publication Aeromonas hydrophila N-(acridin-9-yl)-4-nitrobenzamide
2.4.2.30 0.086
-
 25°C, pH not specified in the publication Aeromonas hydrophila N-[2-oxo-4-(phenylamino)-3,8a-dihydro-2H-chromen-3-yl]acetamide
2.4.2.30 0.157
-
 25°C, pH not specified in the publication Aeromonas hydrophila V8 protease