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Literature summary extracted from

  • Meech, R.; Rogers, A.; Zhuang, L.; Lewis, B.C.; Miners, J.O.; Mackenzie, P.I.
    Identification of residues that confer sugar selectivity to UDP-glycosyltransferase 3A (UGT3A) enzymes (2012), J. Biol. Chem., 287, 24122-24130.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.B7 analysis of homology models docked with UDP-sugar donors indicates that residue N391 in UGT3A1 is able to accommodate the N-acetyl group on C2 of UDP-GlcNAc so that the anomeric carbon atom C1 is optimally situated for catalysis involving residue His35. Replacement of Asn with Phe at position 391 disrupts this catalytically productive orientation of UDP-GlcNAc but allows a more optimal alignment of UDP-Glc for sugar donation Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B7 N391F residue N391 is necessary for utilization of UDP-GlcNAc as substrate. Mutation N391F in UGT3A1 enhances its ability to utilize UDP-Glc and completely inhibits its ability to use UDP-GlcNAc. An analysis of homology models docked with UDP-sugar donors indicates that N391 in UGT3A1 is able to accommodate the N-acetyl group on C2 of UDP-GlcNAc so that the anomeric carbon atom C1 is optimally situated for catalysis involving residue His35. Replacement of Asn with Phe at position 391 disrupts this catalytically productive orientation of UDP-GlcNAc but allows a more optimal alignment of UDP-Glc for sugar donation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.B7 0.00175
-
 UDP-D-glucose wild-type, pH 7.5, 37°C Homo sapiens
2.4.1.B7 0.015
-
 UDP-D-glucose mutant N391F, pH 7.5, 37°C Homo sapiens
2.4.1.B7 0.065
-
 UDP-N-acetyl-D-glucosamine wild-type, pH 7.5, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B7 Homo sapiens
-
-
-
2.4.1.B7 Pan troglodytes
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B7 UDP-D-glucose + ursodeoxycholate
-
 Homo sapiens UDP + 7beta-D-glucosyl-ursodeoxycholate
-
 ?
2.4.1.B7 UDP-N-acetyl-D-glucosamine + ursodeoxycholate
-
 Homo sapiens UDP + 7beta-(N-acetyl-D-glucosaminyl)-ursodeoxycholate
-
 ?
2.4.1.B7 UDP-N-acetyl-D-glucosamine + ursodeoxycholate
-
 Pan troglodytes UDP + 7beta-(N-acetyl-D-glucosaminyl)-ursodeoxycholate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B7 UGT3A1
-
 Homo sapiens
2.4.1.B7 UGT3A1
-
 Pan troglodytes

General Information

EC Number General Information Comment Organism
2.4.1.B7 physiological function multispecies sequence analysis reveals that only primates possess UGT3A sequences containing residue Asn391, suggesting that other mammals may not have the capacity to N-acetylglucosaminidate small molecules. In support of this hypothesis, Asn391-containing UGT3A forms from two non-human primates are found to use UDP-GlcNAc, whereas UGT3A isoforms from non-primates can could not use this sugar donor Homo sapiens
2.4.1.B7 physiological function multispecies sequence analysis reveals that only primates possess UGT3A sequences containing residue Asn391, suggesting that other mammals may not have the capacity to N-acetylglucosaminidate small molecules. In support of this hypothesis, Asn391-containing UGT3A forms from two non-human primates are found to use UDP-GlcNAc, whereas UGT3A isoforms from non-primates can could not use this sugar donor Pan troglodytes