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Literature summary extracted from

  • Cabrera, R.; Baez, M.; Pereira, H.M.; Caniuguir, A.; Garratt, R.C.; Babul, J.
    The crystal complex of phosphofructokinase-2 of Escherichia coli with fructose-6-phosphate: Kinetic and structural analysis of the allosteric ATP inhibition (2011), J. Biol. Chem., 286, 5774-5783.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 ADP
-
 Escherichia coli
2.7.1.11 GDP
-
 Escherichia coli
2.7.1.11 MgATP2- the apparent Km for MgATP2- is 0.022 mM Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.11 isozyme Pfk-2 in complex with D-fructose 6-phosphate, hanging drop vapor diffusion method, using 23% (w/v) PEG 4000, 100 mM sodium acetate, pH 4.75, and 200 mM ammonium acetate Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 D-fructose 6-phosphate inhibition occurs at low concentrations of fructose 6-phosphate Escherichia coli
2.7.1.11 MgATP2- inhibition occurs at high concentrations of MgATP2- Escherichia coli
2.7.1.11 phosphoenolpyruvate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity, bound to ATP Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Escherichia coli P06999
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 homotetramer x-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 Pfk-2 isoform Escherichia coli
2.7.1.11 phosphofructokinase-2 isoform Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.11 44
-
 D-fructose 6-phosphate in the presence of 0.5 mM MgATP2-, at pH 8.2 and 25°C Escherichia coli
2.7.1.11 60
-
 D-fructose 6-phosphate in the presence of 0.1 mM MgATP2-, at pH 8.2 and 25°C Escherichia coli