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Literature summary extracted from

  • Kurata, A.; Kurihara, T.; Kamachi, H.; Esaki N.
    2-Haloacrylate reductase, a novel enzyme of the medium chain dehydrogenase/reductase superfamily that catalyzes the reduction of a carbon-carbon double bond of unsaturated organohalogen compounds (2005), J. Biol. Chem., 280, 20286-20291.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.103 synthesis the enzyme is useful for the production of (S)-2-chloropropionate, which is used for the industrial production of aryloxyphenoxypropionic acid herbicides Burkholderia sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.103 overproduced in Escherichia coli Burkholderia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.103 2-bromoacrylate above 2.5 mM Burkholderia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.103 0.09
-
 NADPH pH 8.0, 35°C Burkholderia sp.
1.3.1.103 0.19
-
 2-bromoacrylate pH 8.0, 35°C Burkholderia sp.
1.3.1.103 0.45
-
 2-chloroacrylate pH 8.0, 35°C Burkholderia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.103 30000
-
 gel filtration Burkholderia sp.
1.3.1.103 35656
-
 1 * 35656, calculated from sequence Burkholderia sp.
1.3.1.103 35660
-
 mass spectrometric analysis Burkholderia sp.
1.3.1.103 37000
-
 1 * 37000, SDS-PAGE Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.103 2-bromoacrylate + NADPH + H+ Burkholderia sp. no activity with NADH (S)-2-bromopropanoate + NADP+
-
 ?
1.3.1.103 2-chloroacrylate + NADPH + H+ Burkholderia sp. the enzyme is involved in the degradation of unsaturated organohalogen compounds (S)-2-chloropropanoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.103 Burkholderia sp. Q59I44
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.103 recombinant enzyme Burkholderia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.103 1.9
-
 2-chloroacrylate, pH 8.0, 35°C Burkholderia sp.
1.3.1.103 2.8
-
 2-bromoacrylate, pH 8.0, 35°C Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.103 2-bromoacrylate + NADPH + H+ no activity with NADH Burkholderia sp. (S)-2-bromopropanoate + NADP+
-
 ?
1.3.1.103 2-chloroacrylate + NADPH + H+ no activity with NADH Burkholderia sp. (S)-2-chloropropanoate + NADP+
-
 ?
1.3.1.103 2-chloroacrylate + NADPH + H+ the enzyme is involved in the degradation of unsaturated organohalogen compounds Burkholderia sp. (S)-2-chloropropanoate + NADP+
-
 ?
1.3.1.103 additional information no activity with 1,4-benzoquinone, 1,4-naphthoquinone, acrylate and methacrylate Burkholderia sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.103 monomer 1 * 37000, SDS-PAGE Burkholderia sp.
1.3.1.103 monomer 1 * 35656, calculated from sequence Burkholderia sp.

Synonyms

EC Number Synonyms Comment Organism
1.3.1.103 CAA43
-
 Burkholderia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.103 35
-
 assay at Burkholderia sp.
1.3.1.103 40
-
-
 Burkholderia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.103 35
-
 stable below, 30 min Burkholderia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.103 8
-
-
 Burkholderia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.103 7 8 30°C, 30 min Burkholderia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.103 NADPH no activity with NADH Burkholderia sp.