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Literature summary extracted from

  • Stieglitz, K.A.; Johnson, K.A.; Yang, H.; Roberts, M.F.; Seaton, B.A.; Head, J.F.; Stec, B.
    Crystal structure of a dual activity IMPase/FBPase (AF2372) from Archaeoglobus fulgidus. The story of a mobile loop (2002), J. Biol. Chem., 277, 22863-22874.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 overexpression in Escherichia coli Archaeoglobus fulgidus
3.1.3.25 overexpression in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.11 crystallized by vapor diffusion using hanging drops of 0.005 ml. The apoenzyme is crystallized in either 0.2 M ammonium nitrate and 30% PEG 3350 or 0.2 M dihydrogen ammonium phosphate and 30% PEG 3350 Archaeoglobus fulgidus
3.1.3.25 crystallized by vapor diffusion using hanging drops of 0.005 ml. The apoenzyme is crystallized in either 0.2 M ammonium nitrate and 30% PEG 3350 or 0.2 M dihydrogen ammonium phosphate and 30% PEG 3350 Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 Ca2+
-
 Archaeoglobus fulgidus
3.1.3.11 Li+
-
 Archaeoglobus fulgidus
3.1.3.25 Ca2+
-
 Archaeoglobus fulgidus
3.1.3.25 Li+
-
 Archaeoglobus fulgidus
3.1.3.25 additional information 300 mM Na+ or K+have no effect Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Archaeoglobus fulgidus O30298
-
-
3.1.3.25 Archaeoglobus fulgidus O30298
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Archaeoglobus fulgidus
3.1.3.25
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Archaeoglobus fulgidus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 myo-inositol phosphate + H2O
-
 Archaeoglobus fulgidus myo-inositol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 AF2372
-
 Archaeoglobus fulgidus
3.1.3.11 IMPase/FBPase
-
 Archaeoglobus fulgidus
3.1.3.11 SuhB
-
 Archaeoglobus fulgidus
3.1.3.25 AF2372
-
 Archaeoglobus fulgidus
3.1.3.25 IMPase/FBPase
-
 Archaeoglobus fulgidus
3.1.3.25 SuhB
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.11 87
-
 Tm-value Archaeoglobus fulgidus
3.1.3.25 87
-
 Tm-value Archaeoglobus fulgidus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.11 290
-
 pH 8.0, 85°C Archaeoglobus fulgidus Li+
3.1.3.25 290
-
 pH 8.0, 85°C Archaeoglobus fulgidus Li+