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Literature summary extracted from

  • Chen, C.D.; Lin, C.H.; Chuankhayan, P.; Huang, Y.C.; Hsieh, Y.C.; Huang, T.F.; Guan, H.H.; Liu, M.Y.; Chang, W.C.; Chen, C.J.
    Crystal structures of complexes of the branched-chain aminotransferase from Deinococcus radiodurans with alpha-ketoisocaproate and L-glutamate suggest the radiation resistance of this enzyme for catalysis (2012), J. Bacteriol., 194, 6206-6216.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 His-tagged protein expressed in Escherichia coli JM109 Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.42 hanging-drop vapor diffusion method, PEG 8000, pH 7.4, 20°C Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.42 K202R active site residue, no activity detected Deinococcus radiodurans
2.6.1.42 R145E no activity detected Deinococcus radiodurans
2.6.1.42 Y71A/R145A no activity detected Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Deinococcus radiodurans Q9RTX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 immobilized metal ion affinity chromatography (Ni2+) Deinococcus radiodurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 additional information
-
 62.5% activity with 2-oxoisovalerate, 34.9% activity with 3-methyl-2-oxovalerate, 22.7% activity with 2-oxo-4-methylthiobutyrate, 57.1% activity with 2-oxohexanoate, 51.0% activity with 2-oxovalerate Deinococcus radiodurans
2.6.1.42 180
-
 substrates 2-oxoisocaproate and L-glutamate, pH 8.3, 25°C Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
 Deinococcus radiodurans 3-methyl-2-oxopentanoic acid + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Deinococcus radiodurans 4-methyl-2-oxopentanoic acid + L-glutamate
-
 r
2.6.1.42 L-valine + 2-oxoglutarate
-
 Deinococcus radiodurans 3-methyl-2-oxobutanoic acid + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.42 homodimer crystal structure Deinococcus radiodurans

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 branched-chain aminotransferase
-
 Deinococcus radiodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.42 60
-
 stable at least for 10 min, about 40% activity after 10 min at 65% Deinococcus radiodurans

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Deinococcus radiodurans