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Literature summary extracted from
- Characterization of two beta-1,3-glucosyltransferases from Escherichia coli serotypes O56 and O152 (2008), J. Bacteriol., 190, 4922-4932.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.305 | - |
Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.305 | CHAPS | 0.5%, 41% inhibition; 0.5%, 75% inhibition | Escherichia coli |  |
| 2.4.1.305 | Co2+ | the addition of 5 mM Co2+ reduces the activation by 5 mM MnCl2 of the enzyme by 45%; the addition of 5 mM Co2+ reduces the activation by 5 mM MnCl2 of the enzyme by 76% | Escherichia coli | |
| 2.4.1.305 | EDTA | - |
Escherichia coli | |
| 2.4.1.305 | octyl-beta-D-glucoside | 0.5%, 15% inhibition; 0.5%, 78% inhibition | Escherichia coli | |
| 2.4.1.305 | Triton X-100 | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.305 | additional information | - |
N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecanol | it is not possible to determine the accurate Km and Vmax values for the standard acceptor substrate D-GlcNAc-alpha-diphospho-(CH2)11-O-Ph due to its hydrophobic character | Escherichia coli | |
| 2.4.1.305 | additional information | - |
N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | it is not possible to determine the accurate Km and Vmax values for the standard acceptor substrate N-acetyl-D-glucosaminyl-alpha-diphospho-11-phenoxyundecaprenol due to its hydrophobic character | Escherichia coli | |
| 2.4.1.305 | 0.14 | - |
UDP-alpha-D-glucose | pH 7.0, 37°C, assayed with bacterial membranes | Escherichia coli | |
| 2.4.1.305 | 0.5 | - |
UDP-alpha-D-glucose | pH 7.0, 37°C, assayed with bacterial membranes | Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.305 | membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.305 | Co2+ | cobalt(II)-acetate supports glucosyltransferase activity but much less than with MnCl2 | Escherichia coli | |
| 2.4.1.305 | Mg2+ | both MnCl2 and MgCl2 are efficient activators at 5 mM concentration | Escherichia coli | |
| 2.4.1.305 | Mn2+ | both MnCl2 and MgCl2 are efficient activators at 5 mM concentration. The enzyme is fully active at MnCl2 concentrations between 0.5 and 25 mM | Escherichia coli | |
| 2.4.1.305 | additional information | no activation by Ni2+, Cu2+, Pb2+, Zn2+, and Ca2+ | Escherichia coli | |
| 2.4.1.305 | additional information | no activation by Ni2+, Cu2+, Pb2+, Zn2+, and Ca2+ significantly supported the glucosyltransferase activities | Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.305 | 55000 | - |
SDS-PAGE | Escherichia coli |
| 2.4.1.305 | 55400 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | Escherichia coli | the enzyme catalyzes the formation of the Glc-(1->3)-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide of Escherichia coli serotype O152 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | Escherichia coli | the enzyme catalyzes the formation of the Glc-1-3-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide Of Escherichia coli serotype O56 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | Escherichia coli serotype O56 | the enzyme catalyzes the formation of the Glc-1-3-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide Of Escherichia coli serotype O56 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | Escherichia coli serotype O152 | the enzyme catalyzes the formation of the Glc-(1->3)-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide of Escherichia coli serotype O152 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.305 | Escherichia coli | B5L3F2 | - |
- |
| 2.4.1.305 | Escherichia coli | Q077R2 | - |
- |
| 2.4.1.305 | Escherichia coli serotype O152 | B5L3F2 | - |
- |
| 2.4.1.305 | Escherichia coli serotype O56 | Q077R2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.305 | purified protein has no enzyme activity. The native enzyme is associated with membranes which may be essential for their function | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.305 | 0.0022 | - |
pH 7.0, 37°C, assayed with bacterial membranes | Escherichia coli |
| 2.4.1.305 | 0.025 | - |
pH 7.0, 37°C, assayed with bacterial membranes | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.305 | -20°C, stable for several weeks | Escherichia coli |
| 2.4.1.305 | 4°C, stable for several days | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.305 | additional information | no activity with N-butyryl-glucosamine-beta-1-thio-2-naphthyl as acceptor substrate | Escherichia coli | ? | - |
? | |
| 2.4.1.305 | additional information | no activity with N-butyryl-glucosamine-beta-1-thio-2-naphthyl as acceptor substrate | Escherichia coli serotype O56 | ? | - |
? | |
| 2.4.1.305 | additional information | no activity with N-butyryl-glucosamine-beta-1-thio-2-naphthyl as acceptor substrate | Escherichia coli serotype O152 | ? | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + alpha-D-GlcNAc-diphospho-(CH2)9-CH3 | 150% of the activity compared to N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol as acceptor substrate | Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-(CH2)9-CH3 | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + alpha-D-GlcNAc-diphospho-(CH2)9-CH3 | 150% of the activity compared to N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol as acceptor substrate | Escherichia coli serotype O152 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-(CH2)9-CH3 | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + alpha-D-GlcNAc-diphospho-16-phenoxyhexadecanol | - |
Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-16-phenoxyhexadecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + alpha-D-GlcNAc-diphospho-16-phenoxyhexadecanol | - |
Escherichia coli serotype O152 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-16-phenoxyhexadecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + alpha-D-GlcNAc-diphospho-6-phenoxyhexanol | - |
Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-6-phenoxyhexanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + alpha-D-GlcNAc-diphospho-6-phenoxyhexanol | - |
Escherichia coli serotype O152 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-6-phenoxyhexanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-(CH2)9-CH3 | 32% of the activity compared to N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol as acceptor substrate | Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-(CH2)9-CH3 | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-(CH2)9-CH3 | 32% of the activity compared to N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol as acceptor substrate | Escherichia coli serotype O56 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-(CH2)9-CH3 | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecanol | the enzyme requires the diphosphate-sugar linkage in the acceptor substrate for full activity. Substrates lacking diphosphate linked to GlcNAc are inactive. An acceptor analog containing a single phosphate group (D-GlcNAc-alpha-diphospho-11-phenoxyundecanol) shows 11% activity. The phenyl group is not required for activity. The length of the aliphatic chain of acceptor substrates also contributes to the activity. The synthetic acceptor N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol is an analog of the natural undecaprenol-pyrophosphate-sugar acceptor substrate. No activity with CMP-sialic acid, UDP-GlcNAc, UDP-GalNAc, or GDP-Man as donor substrate. UDP-Gal shows 0.2% of the activity compared to UDP-D-glucose | Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-11-phenoxyundecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecanol | the enzyme requires the diphosphate-sugar linkage in the acceptor substrate for full activity. Substrates lacking diphosphate linked to GlcNAc are inactive. An acceptor analog containing a single phosphate group (D-GlcNAc-alpha-P-(CH2)11-O-Ph) shows 1% activity. The phenyl group is not required for activity. The length of the aliphatic chain of acceptor substrates also contributes to the activity. The synthetic acceptor N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol is an analog of the natural undecaprenol-diphosphate-sugar acceptor substrate. No activity with CMP-sialic acid, UDP-GlcNAc, UDP-GalNAc, or GDP-Man as donor substrate. UDP-Gal shows 0.6% of the activity compared to UDP-D-glucose | Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-11-phenoxyundecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecanol | the enzyme requires the diphosphate-sugar linkage in the acceptor substrate for full activity. Substrates lacking diphosphate linked to GlcNAc are inactive. An acceptor analog containing a single phosphate group (D-GlcNAc-alpha-P-(CH2)11-O-Ph) shows 1% activity. The phenyl group is not required for activity. The length of the aliphatic chain of acceptor substrates also contributes to the activity. The synthetic acceptor N-acetyl-alpha-D-glucosaminyl-diphospho-11-phenoxyundecaprenol is an analog of the natural undecaprenol-diphosphate-sugar acceptor substrate. No activity with CMP-sialic acid, UDP-GlcNAc, UDP-GalNAc, or GDP-Man as donor substrate. UDP-Gal shows 0.6% of the activity compared to UDP-D-glucose | Escherichia coli serotype O56 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-11-phenoxyundecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-16-phenoxyhexadecanol | - |
Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-16-phenoxyhexadecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-16-phenoxyhexadecanol | - |
Escherichia coli serotype O56 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-16-phenoxyhexadecanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-6-phenoxyhexanol | - |
Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-6-phenoxyhexanol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme catalyzes the formation of the Glc-(1->3)-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide of Escherichia coli serotype O152 | Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme catalyzes the formation of the Glc-1-3-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide Of Escherichia coli serotype O56 | Escherichia coli | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme catalyzes the formation of the Glc-1-3-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide Of Escherichia coli serotype O56 | Escherichia coli serotype O56 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
| 2.4.1.305 | UDP-alpha-D-glucose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme catalyzes the formation of the Glc-(1->3)-GlcNAc linkage in the repeating units of the O-antigen oligosaccharide of Escherichia coli serotype O152 | Escherichia coli serotype O152 | UDP + beta-D-Glc-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.305 | ? | x * 55000, SDS-PAGE | Escherichia coli |
| 2.4.1.305 | ? | x * 55400, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.305 | UDP-Glc:GlcNAc-pyrophosphate-lipid beta-1,3-glucosyltransferase | - |
Escherichia coli |
| 2.4.1.305 | WfaP | - |
Escherichia coli |
| 2.4.1.305 | WfgD | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.305 | 37 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.305 | 6 | - |
- |
Escherichia coli |
| 2.4.1.305 | 6.5 | - |
- |
Escherichia coli |
| 2.4.1.305 | 7 | - |
assay at | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.305 | 5 | 9 | pH 5.0: about 60% of maximal activity, pH 9.0: about 40% of maximal activity | Escherichia coli |
| 2.4.1.305 | 5 | 9 | pH 5.0: about 75% of maximal activity, pH 9.0: about 75% of maximal activity | Escherichia coli |
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