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Literature summary extracted from

  • Feinberg, L.F.; Holden, J.F.
    Characterization of dissimilatory Fe(III) versus NO3- reduction in the hyperthermophilic archaeon Pyrobaculum aerophilum (2006), J. Bacteriol., 188, 525-531.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.1.10 cytoplasm
-
 Pyrobaculum aerophilum 5737
-
1.16.1.10 membrane
-
 Pyrobaculum aerophilum 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.10 Pyrobaculum aerophilum
-
-
-
1.16.1.10 Pyrobaculum aerophilum DSM 7523
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.1.10 culture condition:ferric citrate-grown cell NADH-dependent ferric reductase activity increases approximately 100fold in whole-cell extracts from iron-grown cells relative to those from nitrate-grown cells Pyrobaculum aerophilum
-
1.16.1.10 culture condition:nitrate-grown cell NADH-dependent ferric reductase activity increases approximately 100fold in whole-cell extracts from iron-grown cells relative to those from nitrate-grown cells Pyrobaculum aerophilum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.10 2 Fe(III)-ferrozine + NADPH
-
 Pyrobaculum aerophilum 2 Fe(II)-ferrozine + NADP+ + H+
-
 ?
1.16.1.10 2 Fe(III)-ferrozine + NADPH
-
 Pyrobaculum aerophilum DSM 7523 2 Fe(II)-ferrozine + NADP+ + H+
-
 ?
1.16.1.10 2 Fe3+ + 3 ferrozine + NADH
-
 Pyrobaculum aerophilum 2 Fe(II)-ferrozine + NAD+ + H+
-
 ?
1.16.1.10 2 Fe3+ + 3 ferrozine + NADH
-
 Pyrobaculum aerophilum DSM 7523 2 Fe(II)-ferrozine + NAD+ + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.16.1.10 80
-
 assay at Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.16.1.10 6.5
-
 assay at Pyrobaculum aerophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.10 NADH rates with NADPH as the electron donor are not significantly different from those with NADH as the donor, activity in cell extract, enzyme not purified Pyrobaculum aerophilum
1.16.1.10 NADPH rates with NADPH as the electron donor are not significantly different from those with NADH as the donor, activity in cell extract, enzyme not purified Pyrobaculum aerophilum

General Information

EC Number General Information Comment Organism
1.16.1.10 physiological function dissimilatory reduction of iron Pyrobaculum aerophilum