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Literature summary extracted from
- A hyperactive NAD(P)H:rubredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus (1999), J. Bacteriol., 181, 5530-5533.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.1.4 | FAD | the enzyme contains one flavin adenine dinucleotide (FAD) molecule per mol | Pyrococcus furiosus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.18.1.4 | 0.005 | - |
NADPH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
| 1.18.1.4 | 0.01 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus | |
| 1.18.1.4 | 0.034 | - |
NADH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
| 1.18.1.4 | 0.05 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate: NADPH | Pyrococcus furiosus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.1.4 | additional information | no metals can be detected | Pyrococcus furiosus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.4 | 45000 | - |
1 * 45000, SDS-PAGE | Pyrococcus furiosus |
| 1.18.1.4 | 51000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.1.4 | 2 oxidized rubredoxin + NADH | Pyrococcus furiosus | - |
2 reduced rubredoxin + NAD+ + H+ | - |
? | |
| 1.18.1.4 | 2 oxidized rubredoxin + NADPH | Pyrococcus furiosus | rubredoxin is the most efficient electron acceptor | 2 reduced rubredoxin + NADP+ + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.18.1.4 | Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.18.1.4 | - |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.4 | 20000 | - |
pH 8.0, 80°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.1.4 | 2 oxidized rubredoxin + NADH | - |
Pyrococcus furiosus | 2 reduced rubredoxin + NAD+ + H+ | - |
? | |
| 1.18.1.4 | 2 oxidized rubredoxin + NADPH | rubredoxin is the most efficient electron acceptor | Pyrococcus furiosus | 2 reduced rubredoxin + NADP+ + H+ | - |
? | |
| 1.18.1.4 | additional information | the enzyme does not reduce Pyrococcus furiosus ferredoxin | Pyrococcus furiosus | ? | - |
? | |
| 1.18.1.4 | oxidized benzyl viologen + NADH | - |
Pyrococcus furiosus | reduced benzyl viologen + NAD+ | - |
? | |
| 1.18.1.4 | oxidized benzyl viologen + NADPH | - |
Pyrococcus furiosus | reduced benzyl viologen + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.18.1.4 | monomer | 1 * 45000, SDS-PAGE | Pyrococcus furiosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.18.1.4 | NROR | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.4 | 80 | - |
assay at | Pyrococcus furiosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.4 | 25 | 80 | about 70fold increase in kcat/Km for oxidized rebredoxin increases from 25°C to 80°C | Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.1.4 | 80 | - |
12 h, 50% loss of activity | Pyrococcus furiosus |
| 1.18.1.4 | 95 | - |
2 h, 50% loss of activity | Pyrococcus furiosus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.18.1.4 | 221 | - |
oxidized rubredoxin | pH 8.0, 25°C, cosubstrate NADPH | Pyrococcus furiosus | |
| 1.18.1.4 | 341 | - |
NADPH | pH 8.0, 80°C, cosubstrate: benzyl viologen | Pyrococcus furiosus | |
| 1.18.1.4 | 354 | - |
NADH | pH 8.0, 80°C, cosubstrate: benzyl viologen | Pyrococcus furiosus | |
| 1.18.1.4 | 15000 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.18.1.4 | 10400 | - |
NADH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
| 1.18.1.4 | 22050 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus | |
| 1.18.1.4 | 68300 | - |
NADPH | pH 8.0, 80°C, cosubstrate benzyl viologen | Pyrococcus furiosus | |
| 1.18.1.4 | 300000 | - |
oxidized rubredoxin | pH 8.0, 80°C, cosubstrate NADPH | Pyrococcus furiosus |
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