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Literature summary extracted from

  • Mukund, S.; Adams, M.W.
    Molybdenum and vanadium do not replace tungsten in the catalytically active forms of the three tungstoenzymes in the hyperthermophilic archaeon Pyrococcus furiosus (1996), J. Bacteriol., 178, 163-167.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.B2 Tungsten the enzyme from Pyrococcus furiosus uses exclusively tungsten to synthesize the catalytically active forms of formaldehyde ferredoxin oxidoreductase and active molybdenum- or vanadium-containing isoenzymes are not expressed when the cells are grown in the presence of these other metals Pyrococcus furiosus
1.2.7.5 Tungsten the enzyme from Pyrococcus furiosus uses exclusively tungsten to synthesize the catalytically active forms of aldehyde ferredoxin oxidoreductase and active molybdenum- or vanadium-containing isoenzymes are not expressed when the cells are grown in the presence of these other metals Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus
-
-
-
1.2.7.5 Pyrococcus furiosus Q51739
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.B2 enzyme from vanadium- and molybdenum-grown cells Pyrococcus furiosus
1.2.7.5 enzyme from vanadium- and molybdenum-grown cells Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.B2 formaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus crotonate + H+ + reduced benzyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.B2 FOR
-
 Pyrococcus furiosus
1.2.7.5 AOR
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.B2 80
-
 assay at Pyrococcus furiosus
1.2.7.5 80
-
 assay at Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.B2 8.4
-
 assay at Pyrococcus furiosus
1.2.7.5 8.4
-
 assay at Pyrococcus furiosus