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Literature summary extracted from

  • Yoshimoto, A.; Ogasawara, T.; Kitamura, I.; Oki, T.; Inui, T.; Takeuchi, T.; Umezawa, H.
    Enzymatic conversion of aclacinomycin A to Y by a specific oxidoreductase in Streptomyces (1979), J. Antibiot., 32, 472-481.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.3.14 no loss of activity is observed on freezing Streptomyces galilaeus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.14 ascorbic acid 5 mM, 44% inhibition Streptomyces galilaeus
1.3.3.14 Fe2+ 0.5 mM, 26% inhibition. 1 mM, 73% inhibition Streptomyces galilaeus
1.3.3.14 NaN3 1 mM, 15% inhibition. 5 mM, 53% inhibition Streptomyces galilaeus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.14 72000
-
 gel filtration Streptomyces galilaeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.14 aclacinomycin A + O2 Streptomyces galilaeus
-
 aclacinomycin Y + H2O2
-
 ?
1.3.3.14 aclacinomycin A + O2 Streptomyces galilaeus MA144-M1 6U-21
-
 aclacinomycin Y + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.14 Streptomyces galilaeus
-
-
-
1.3.3.14 Streptomyces galilaeus MA144-M1 6U-21
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.14
-
 Streptomyces galilaeus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.14 952
-
 pH and temperature not specified in the publication Streptomyces galilaeus

Storage Stability

EC Number Storage Stability Organism
1.3.3.14 -20°C, in 10% glycerol, 0.05 M Tris-HCl, pH 7.2, stable for at least 2 months Streptomyces galilaeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.14 aclacinomycin A + O2
-
 Streptomyces galilaeus aclacinomycin Y + H2O2
-
 ?
1.3.3.14 aclacinomycin A + O2 the enzyme catalyzes the oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. NAD+, NADP+, phenazine methosulfate and 2,6-dichlorophenolindophenol can not serve as electron acceptors Streptomyces galilaeus aclacinomycin Y + H2O2
-
 ?
1.3.3.14 aclacinomycin A + O2
-
 Streptomyces galilaeus MA144-M1 6U-21 aclacinomycin Y + H2O2
-
 ?
1.3.3.14 aclacinomycin A + O2 the enzyme catalyzes the oxidation of the terminal sugar, L-cinerulose, of the trisaccharide moiety of aclacinomycin A to L-aculose (2,3,6-trideoxyhex-2-enopyranos-4-ulose) with removal of two electrons. NAD+, NADP+, phenazine methosulfate and 2,6-dichlorophenolindophenol can not serve as electron acceptors Streptomyces galilaeus MA144-M1 6U-21 aclacinomycin Y + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.14 50
-
 pH 7, 30 min, stable Streptomyces galilaeus
1.3.3.14 60
-
 pH 7, 30 min, 20% loss of activity Streptomyces galilaeus
1.3.3.14 80
-
 pH 7, 30 min, 50% loss of activity Streptomyces galilaeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.14 5.5
-
-
 Streptomyces galilaeus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.14 3.8 7 pH 3.8: about 40% of maximal activity, pH 7.0: about 65% of maximal activity Streptomyces galilaeus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.14 5 8 37°C, 3 h, stable Streptomyces galilaeus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.3.14 Streptomyces galilaeus isoelectric focusing
-
 5.9