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Literature summary extracted from

  • Yi, W.; Shao, J.; Zhu, L.; Li, M.; Singh, M.; Lu, Y.; Lin, S.; Li, H.; Ryu, K.; Shen, J.; Guo, H.; Yao, Q.; Bush, C.A.; Wang, P.G.
    Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide (2005), J. Am. Chem. Soc., 127, 2040-2041.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.308 synthesis stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing us an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases Escherichia coli
2.4.1.309 synthesis stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.308 expressed in Escherichia coli as a GST-tagged fusion protein Escherichia coli
2.4.1.309 expressed in Escherichia coli as a GST-tagged fusion protein Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.122 Escherichia coli Q4KXC9
-
-
2.4.1.122 Escherichia coli O86:B7 Q4KXC9
-
-
2.4.1.308 Escherichia coli Q58YV9
-
-
2.4.1.308 Escherichia coli O86 Q58YV9
-
-
2.4.1.309 Escherichia coli Q5JBG6
-
-
2.4.1.309 Escherichia coli O86 Q5JBG6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.122 UDP-alpha-D-galactose + alpha-D-GalNAc-OMe
-
 Escherichia coli UDP + alpha-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.122 UDP-alpha-D-galactose + alpha-D-GalNAc-OMe
-
 Escherichia coli O86:B7 UDP + alpha-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 Escherichia coli GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 Escherichia coli O86 GDP + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-OMe 15% of the activity compared to beta-Gal-(1->3)-alpha-GalNAc-OMe Escherichia coli ?
-
 ?
2.4.1.308 GDP-beta-L-fucose + beta-D-Gal-OMe 15% of the activity compared to beta-Gal-(1->3)-alpha-GalNAc-OMe Escherichia coli O86 ?
-
 ?
2.4.1.308 additional information WbnK requires a terminal beta-1,3-linked Gal sugar sequence as acceptor Escherichia coli ?
-
 ?
2.4.1.308 additional information WbnK requires a terminal beta-1,3-linked Gal sugar sequence as acceptor Escherichia coli O86 ?
-
 ?
2.4.1.309 additional information WbnI prefers terminal fucosylated sugar structures to nonfucosylated ones Escherichia coli ?
-
 ?
2.4.1.309 additional information WbnI prefers terminal fucosylated sugar structures to nonfucosylated ones Escherichia coli O86 ?
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 Escherichia coli UDP + alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 Escherichia coli O86 UDP + alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-OMe 30% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe Escherichia coli ?
-
 ?
2.4.1.309 UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-OMe 30% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe Escherichia coli O86 ?
-
 ?
2.4.1.309 UDP-alpha-D-galactose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe 12% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe Escherichia coli alpha-D-Gal-(1->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?
2.4.1.309 UDP-alpha-D-galactose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe 12% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe Escherichia coli O86 alpha-D-Gal-(1->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.122 WbnJ
-
 Escherichia coli
2.4.1.308 WbnK
-
 Escherichia coli
2.4.1.309 WbnI
-
 Escherichia coli