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Literature summary extracted from

  • Gao, Y.S.; Zhao, T.J.; Chen, Z.; Li, C.; Wang, Y.; Yan, Y.B.; Zhou, H.M.
    Isoenzyme-specific thermostability of human cytosolic creatine kinase (2010), Int. J. Biol. Macromol., 47, 27-32.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.2 expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.3
-
 ATP hybrid form consisting of muscle and brain creatine kinase isoforms, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 0.35
-
 ATP wild-type hBBCK, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 0.44
-
 ATP wild-type hMMCK, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 3.9
-
 Creatine wild-type hBBCK, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 5
-
 Creatine hybrid form consisting of muscle and brain creatine kinase isoforms, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 6.2
-
 Creatine wild-type hMMCK, pH and temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.2 85300
-
 wild-type hMMCK Homo sapiens
2.7.3.2 86200
-
 wild-type hMMCK Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 brain
-
 Homo sapiens
-
2.7.3.2 muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Homo sapiens ADP + phosphocreatine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.3.2 brain creatine kinase
-
 Homo sapiens
2.7.3.2 hBBCK
-
 Homo sapiens
2.7.3.2 hMMCK
-
 Homo sapiens
2.7.3.2 muscle creatine kinase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.3.2 42.2
-
 T0.5: 42.2°C wild-type hBBCK Homo sapiens
2.7.3.2 48
-
 wild-type hBBCK gets completely inactivated above 48°C Homo sapiens
2.7.3.2 52
-
 T0.5: 52°C hybrid form consisting of muscle and brain creatine kinase isoforms Homo sapiens
2.7.3.2 57.2
-
 T0.5: 57.2°C wild-type hMMCK Homo sapiens
2.7.3.2 62
-
 wild-type hMMCK gets completely inactivated above 62°C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.2 138
-
 Creatine co-substrate: ATP, wild-type hMMCK, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 238
-
 Creatine co-substrate: ATP, hybrid form consisting of muscle and brain creatine kinase isoforms, pH and temperature not specified in the publication Homo sapiens
2.7.3.2 417
-
 Creatine co-substrate: ATP, wild-type hBBCK, pH and temperature not specified in the publication Homo sapiens