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Literature summary extracted from

  • Seelhorst, K.; Stacke, C.; Ziegelmueller, P.; Hahn, U.
    N-Glycosylations of human alpha1,3-fucosyltransferase IX are required for full enzyme activity (2013), Glycobiology, 23, 559-567.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 applying a baculovirus system in Spodoptera frugiperda Sf9 cells Homo sapiens
2.4.1.152 expressed in Sf9 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.152 N101Q the mutant shows about 30% of wild type activity Homo sapiens
2.4.1.152 N101Q the mutant still shows about 27% of wild type activity Homo sapiens
2.4.1.152 N101Q/N153Q the mutations lead to an almost complete loss of enzymatic activity Homo sapiens
2.4.1.152 N153Q the mutant almost completely loses enzymatic activity Homo sapiens
2.4.1.152 N153Q the mutation leads to an almost complete loss of enzymatic activity Homo sapiens
2.4.1.152 N191Q/N153Q the mutant almost completely loses enzymatic activity Homo sapiens
2.4.1.152 N62Q the mutant shows about 60% of wild type activity Homo sapiens
2.4.1.152 N62Q the mutant still shows about 62% of wild type activity Homo sapiens
2.4.1.152 N62Q/N101Q the mutant almost completely loses enzymatic activity Homo sapiens
2.4.1.152 N62Q/N101Q the mutations lead to an almost complete loss of enzymatic activity Homo sapiens
2.4.1.152 N62Q/N153Q the mutant almost completely loses enzymatic activity Homo sapiens
2.4.1.152 N62Q/N153Q the mutations lead to an almost complete loss of enzymatic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.152 0.0026
-
 GDP-beta-L-fucose wild type enzyme, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
2.4.1.152 0.0026
-
 GDP-beta-L-fucose recombinant wild type enzyme, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.0047
-
 GDP-beta-L-fucose mutant enzyme N101Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
2.4.1.152 0.0047
-
 GDP-beta-L-fucose mutant enzyme N101Q, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.0061
-
 GDP-beta-L-fucose mutant enzyme N62Q, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.0066
-
 GDP-beta-L-fucose mutant enzyme N62Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
2.4.1.152 0.22
-
 N-acetyllactosamine mutant enzyme N101Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
2.4.1.152 0.22
-
 N-acetyllactosamine mutant enzyme N101Q, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.27
-
 N-acetyllactosamine mutant enzyme N62Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
2.4.1.152 0.27
-
 N-acetyllactosamine mutant enzyme N62Q, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.61
-
 N-acetyllactosamine wild type enzyme, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
2.4.1.152 0.61
-
 N-acetyllactosamine recombinant wild type enzyme, at pH 4.5 and 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.152 41000
-
 x * 41000, fully glycosylated His-tagged enzyme, SDS-PAGE Homo sapiens
2.4.1.152 41000
-
 x * 41000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.152 GDP-beta-L-fucose + N-acetyllactosamine Homo sapiens
-
 GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.152 glycoprotein the enzyme contains three putative N-glycosylation sites (Asn62, Asn101 and Asn153) Homo sapiens
2.4.1.152 glycoprotein the enzyme contains three potential N-glycosylation sites (Asn-XSer/Thr, X is any amino acid except proline) at amino acid positions 62, 101 and 153, respectively Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.152 Ni-NTA column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.152 0.067
-
 mutant enzyme N101Q, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.154
-
 mutant enzyme N62Q, at pH 4.5 and 37°C Homo sapiens
2.4.1.152 0.248
-
 wild type enzyme, at pH 4.5 and 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-beta-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.152 GDP-beta-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.152 ? x * 41000, fully glycosylated His-tagged enzyme, SDS-PAGE Homo sapiens
2.4.1.152 ? x * 41000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 alpha1,3-fucosyltransferase IX
-
 Homo sapiens
2.4.1.152 alpha1,3-FucTIX
-
 Homo sapiens
2.4.1.152 FucT IX
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.152 0.042
-
 N-acetyllactosamine mutant enzyme N101Q, at pH 4.5 and 37°C C Homo sapiens
2.4.1.152 0.12
-
 N-acetyllactosamine mutant enzyme N62Q, at pH 4.5 and 37°C C Homo sapiens