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Literature summary extracted from

  • Marino, K.; Guether, M.L.; Wernimont, A.K.; Amani, M.; Hui, R.; Ferguson, M.A.
    Identification, subcellular localization, biochemical properties, and high-resolution crystal structure of Trypanosoma brucei UDP-glucose pyrophosphorylase (2010), Glycobiology, 20, 1619-1630.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.9 purified recombinant enzyme, 17 mg/mL protein is mixed 1:1 with a crystallization buffer of 22% PEG 3350, 0.1 M ammonium sulfate, and 0.1 M Bis-Tris, pH 5.5, X-ray diffraction structure determination and analysis at 1.92 A resolution Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 additional information
-
 additional information Michaelis-Menten kinetics, overview Trypanosoma brucei
2.7.7.9 0.053
-
 UTP pH 7.2, temperature not specified in the publication, recombinant enzyme Trypanosoma brucei
2.7.7.9 0.122
-
 alpha-D-glucose 1-phosphate pH 7.2, temperature not specified in the publication, recombinant enzyme Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.9 glycosome
-
 Trypanosoma brucei 20015
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Co2+ activates Trypanosoma brucei
2.7.7.9 Mg2+ required, best at 10 mM Trypanosoma brucei
2.7.7.9 Mn2+ activates Trypanosoma brucei
2.7.7.9 additional information the enzyme is divalent cation-dependent, no activation by Zn2+ and Cu2+ Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 54507
-
 x * 54507, sequence calculation Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Trypanosoma brucei
-
 diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Trypanosoma brucei 427
-
 diphosphate + UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Trypanosoma brucei D3HTY9
-
-
2.7.7.9 Trypanosoma brucei 427 D3HTY9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 recombinant His6-tagged enzyme from Escherichia coli strain by nickel affinity chromatography and gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 additional information the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate Trypanosoma brucei ?
-
 ?
2.7.7.9 additional information the enzyme is specific for alpha-D-glucose 1-phosphate, no activity with N-acetylgalactosamine 1-phosphate, N-acetylglucosamine 1-phosphate, or galactosamine 1-phosphate Trypanosoma brucei 427 ?
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Trypanosoma brucei diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Trypanosoma brucei 427 diphosphate + UDP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.9 ? x * 54507, sequence calculation Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UDP-glucose pyrophosphorylase
-
 Trypanosoma brucei
2.7.7.9 UGP
-
 Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 6 7.5 forward reaction Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.7.7.9 metabolism the enzyme catalyzes the last and reversible step of the de novo UDP-Glc biosynthetic pathway Trypanosoma brucei
2.7.7.9 additional information UDP-Glc is bound in a deep cleft located at the center of the catalytic domain that consists of highly conserved residues Trypanosoma brucei
2.7.7.9 physiological function UDP-glucose is the onyl precuror for UDP-galactose synthesis, which is one of the sugar nucleotides essentially required for the parasite's survival and infectivity. UDP-glucose is also the glucosyl donor for the unfolded glycoprotein glucosyltransferase involved in glycoprotein quality control in the endoplasmic reticulum and is the presumed donor for the synthesis of base J, i.e. beta-D-glucosyl-hydroxymethyluracil, a rare deoxynucleotide found in telomereproximal DNA in the bloodstream form of the parasite Trypanosoma brucei