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Literature summary extracted from

  • Bhargava, P.; Kumar, K.; Chaudhaery, S.S.; Saxena, A.K.; Roy, U.
    Cloning, overexpression and characterization of Leishmania donovani squalene synthase (2010), FEMS Microbiol. Lett., 311, 82-92.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.21 drug development LdSSN is a potential drug target for visceral leishmaniasis Leishmania donovani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.21 LdSSN DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression as C-terminally His6-tagged protein in Escherichia coli strain BL21 (DE3) Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.21 Guanidinium chloride loss of 86% activity at 0.3M GdmCl, 50% at 0.2 M Leishmania donovani
2.5.1.21 Urea loss of 81% activity at 2 M, 50% at 1 M Leishmania donovani
2.5.1.21 zaragozic acid A is a potent inhibitor of mammalian SSN and also a competitive inhibitor of recombinant Leishmania donovani SSN, 50% inhibition at 100 nM Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.21 additional information
-
 additional information kinetics, recombinant LdSSN, overview Leishmania donovani
2.5.1.21 0.0038
-
 (2E,6E)-farnesyl diphosphate pH 7.4, 37°C Leishmania donovani
2.5.1.21 0.04323
-
 NADPH pH 7.4, 37°C Leishmania donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.21 Mg2+ required Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.21 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Leishmania donovani overall reaction squalene + 2 diphosphate + NADP+
-
 ?
2.5.1.21 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Leishmania donovani MHOM/IN/80/Dd8 overall reaction squalene + 2 diphosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.21 Leishmania donovani Q257D4
-
-
2.5.1.21 Leishmania donovani MHOM/IN/80/Dd8 Q257D4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.21 recombinant His6-tagged LdSSN from Escherichia coli strain BL21(DE3) by anion exchange chromatography, nickel affinity chrmatography is not successful Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.21 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ overall reaction Leishmania donovani squalene + 2 diphosphate + NADP+
-
 ?
2.5.1.21 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ overall reaction Leishmania donovani MHOM/IN/80/Dd8 squalene + 2 diphosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.21 More sequence comparison, overview Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.5.1.21 SSN
-
 Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.21 37
-
-
 Leishmania donovani

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.21 30 45 83% and 88% activity at a temperature of 30°C and 45°C Leishmania donovani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.21 60
-
 purified recombinant enzyme, pH 7.4, loss of 85% activity Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.21 7.4
-
-
 Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.21 6 9 activity range, over 90% of maximal activity at pH 7.0-8.0, 30% at pH 6.0, 20% at pH 9.0 Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.21 NAD(P)H
-
 Leishmania donovani

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.21 0.000074
-
 zaragozic acid A pH 7.4, 37°C Leishmania donovani

General Information

EC Number General Information Comment Organism
2.5.1.21 metabolism squalene synthase is a major enzyme in the sterol biosynthetic pathway Leishmania donovani
2.5.1.21 physiological function squalene synthase catalyses an unusual head-to-head reductive dimerization of two molecules of farnesyl-pyrophosphate in a two-step reaction to form squalene Leishmania donovani