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Literature summary extracted from

  • Chu, H.M.; Chen, F.Y.; Ko, T.P.; Wang, A.H.
    Binding and catalysis of Humulus lupulus adenylate isopentenyltransferase for the synthesis of isopentenylated diadenosine polyphosphates (2010), FEBS Lett., 584, 4083-4088.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.112 expressed in Escherichia coli BL21(DE3) cells Humulus lupulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.27 additional information Humulus lupulus the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + ADP Humulus lupulus
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP Humulus lupulus
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Humulus lupulus
-
-
-
2.5.1.112 Humulus lupulus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + ATP
-
 Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine triphosphate
-
 ?
2.5.1.27 dimethylallyl diphosphate + P1,P3-bis(5'-adenosyl)triphosphate
-
 Humulus lupulus ?
-
 ?
2.5.1.27 dimethylallyl diphosphate + P1,P4-bis(5'-adenosyl)tetraphosphate
-
 Humulus lupulus ?
-
 ?
2.5.1.27 dimethylallyl diphosphate + P1,P5-bis(5'-adenosyl)pentaphosphate
-
 Humulus lupulus ?
-
 ?
2.5.1.27 dimethylallyl diphosphate + P1,P6-bis(5'-adenosyl)hexaphosphate
-
 Humulus lupulus ?
-
 ?
2.5.1.27 additional information the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor Humulus lupulus ?
-
 ?
2.5.1.27 additional information no activity with NAD+, NADP+, and FAD Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + ADP
-
 Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP
-
 Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP ATP is the best cosubstrate Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + diadenosine hexaphosphate
-
 Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + diadenosine pentaphosphate diadenosine pentaphosphate exhibits the highest binding affinity Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + diadenosine tetraphosphate
-
 Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + diadenosine triphosphate diadenosine pentaphosphate exhibits the lowest binding affinity and specific activity compared to ATP Humulus lupulus ?
-
 ?
2.5.1.112 additional information no activity with NAD+, NADP+ or FAD Humulus lupulus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 adenylate isopentenyltransferase
-
 Humulus lupulus
2.5.1.27 AIPT
-
 Humulus lupulus
2.5.1.112 adenylate isopentenyltransferase
-
 Humulus lupulus
2.5.1.112 IPT
-
 Humulus lupulus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.27 25
-
 assay at Humulus lupulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.27 5 7 assay at Humulus lupulus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.112 ADP
-
 Humulus lupulus
2.5.1.112 ATP
-
 Humulus lupulus

General Information

EC Number General Information Comment Organism
2.5.1.27 metabolism cytokinins are important adenine-base phytohormones which promote cell division and their biosynthesis has two pathways depending on different substrates. The first pathway begins with the transfer of isopentenyl group from dimethylallyl diphosphate to the N6-amino group of AMP, ADP or ATP by adenylate isopentenyltransferase, AIPT Humulus lupulus
2.5.1.27 physiological function the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis that transfers the isopentenyl group from dimethylallyl diphosphate to the N6-amino group of adenine Humulus lupulus
2.5.1.112 metabolism the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis Humulus lupulus