BRENDA - Enzyme Database

Binding and catalysis of Humulus lupulus adenylate isopentenyltransferase for the synthesis of isopentenylated diadenosine polyphosphates

Chu, H.M.; Chen, F.Y.; Ko, T.P.; Wang, A.H.; FEBS Lett. 584, 4083-4088 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.5.1.112
expressed in Escherichia coli BL21(DE3) cells
Humulus lupulus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.27
additional information
Humulus lupulus
the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor
?
-
-
-
2.5.1.112
dimethylallyl diphosphate + ADP
Humulus lupulus
-
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
?
2.5.1.112
dimethylallyl diphosphate + ATP
Humulus lupulus
-
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.27
Humulus lupulus
-
-
-
2.5.1.112
Humulus lupulus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.27
dimethylallyl diphosphate + ATP
-
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine triphosphate
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P3-bis(5'-adenosyl)triphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P4-bis(5'-adenosyl)tetraphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P5-bis(5'-adenosyl)pentaphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P6-bis(5'-adenosyl)hexaphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
additional information
the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor
722266
Humulus lupulus
?
-
-
-
-
2.5.1.27
additional information
no activity with NAD+, NADP+, and FAD
722266
Humulus lupulus
?
-
-
-
-
2.5.1.112
dimethylallyl diphosphate + ADP
-
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + ATP
-
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + ATP
ATP is the best cosubstrate
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine hexaphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine pentaphosphate
diadenosine pentaphosphate exhibits the highest binding affinity
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine tetraphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine triphosphate
diadenosine pentaphosphate exhibits the lowest binding affinity and specific activity compared to ATP
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
additional information
no activity with NAD+, NADP+ or FAD
722266
Humulus lupulus
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.27
25
-
assay at
Humulus lupulus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.27
5
7
assay at
Humulus lupulus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.5.1.112
ADP
-
Humulus lupulus
2.5.1.112
ATP
-
Humulus lupulus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.112
expressed in Escherichia coli BL21(DE3) cells
Humulus lupulus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.5.1.112
ADP
-
Humulus lupulus
2.5.1.112
ATP
-
Humulus lupulus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.5.1.27
additional information
Humulus lupulus
the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor
?
-
-
-
2.5.1.112
dimethylallyl diphosphate + ADP
Humulus lupulus
-
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
?
2.5.1.112
dimethylallyl diphosphate + ATP
Humulus lupulus
-
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.27
dimethylallyl diphosphate + ATP
-
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine triphosphate
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P3-bis(5'-adenosyl)triphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P4-bis(5'-adenosyl)tetraphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P5-bis(5'-adenosyl)pentaphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
dimethylallyl diphosphate + P1,P6-bis(5'-adenosyl)hexaphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.27
additional information
the plant AIPT predominantly uses ATP or ADP as the isopentenyl acceptor
722266
Humulus lupulus
?
-
-
-
-
2.5.1.27
additional information
no activity with NAD+, NADP+, and FAD
722266
Humulus lupulus
?
-
-
-
-
2.5.1.112
dimethylallyl diphosphate + ADP
-
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + ATP
-
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + ATP
ATP is the best cosubstrate
722266
Humulus lupulus
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine hexaphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine pentaphosphate
diadenosine pentaphosphate exhibits the highest binding affinity
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine tetraphosphate
-
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
dimethylallyl diphosphate + diadenosine triphosphate
diadenosine pentaphosphate exhibits the lowest binding affinity and specific activity compared to ATP
722266
Humulus lupulus
?
-
-
-
?
2.5.1.112
additional information
no activity with NAD+, NADP+ or FAD
722266
Humulus lupulus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.27
25
-
assay at
Humulus lupulus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.27
5
7
assay at
Humulus lupulus
General Information
EC Number
General Information
Commentary
Organism
2.5.1.27
metabolism
cytokinins are important adenine-base phytohormones which promote cell division and their biosynthesis has two pathways depending on different substrates. The first pathway begins with the transfer of isopentenyl group from dimethylallyl diphosphate to the N6-amino group of AMP, ADP or ATP by adenylate isopentenyltransferase, AIPT
Humulus lupulus
2.5.1.27
physiological function
the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis that transfers the isopentenyl group from dimethylallyl diphosphate to the N6-amino group of adenine
Humulus lupulus
2.5.1.112
metabolism
the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis
Humulus lupulus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.5.1.27
metabolism
cytokinins are important adenine-base phytohormones which promote cell division and their biosynthesis has two pathways depending on different substrates. The first pathway begins with the transfer of isopentenyl group from dimethylallyl diphosphate to the N6-amino group of AMP, ADP or ATP by adenylate isopentenyltransferase, AIPT
Humulus lupulus
2.5.1.27
physiological function
the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis that transfers the isopentenyl group from dimethylallyl diphosphate to the N6-amino group of adenine
Humulus lupulus
2.5.1.112
metabolism
the isopentenyltransferase reaction is the key rate-limiting step in cytokinin biosynthesis
Humulus lupulus