EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.186 | beta-sitosterol | - |
Hellenia speciosa | |
3.2.1.186 | cholesterol | - |
Hellenia speciosa | |
3.2.1.186 | conduritol beta-epoxide | - |
Hellenia speciosa | |
3.2.1.186 | D-glucono-1,5-lactone | 0.25 mM, 50% inhibition | Hellenia speciosa | |
3.2.1.186 | diosgenin | 0.079, 50% inhibition | Hellenia speciosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.186 | 0.05 | - |
protogracillin | pH 5.0, 40°C | Hellenia speciosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.186 | 54000 | - |
1 * 54000 + 1 * 58000, SDS-PAGE | Hellenia speciosa |
3.2.1.186 | 58000 | - |
1 * 54000 + 1 * 58000, SDS-PAGE | Hellenia speciosa |
3.2.1.186 | 110000 | - |
gel filtration | Hellenia speciosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.186 | additional information | Hellenia speciosa | the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.186 | Hellenia speciosa | Q42707 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.186 | - |
Hellenia speciosa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.186 | rhizome | - |
Hellenia speciosa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.186 | 325 | - |
pH 5.0, 40°C | Hellenia speciosa |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.186 | 4°C, 20 mM potassium phosphate buffer, pH 7.0, stable for 3 weeks | Hellenia speciosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.186 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Hellenia speciosa | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.186 | additional information | the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone | Hellenia speciosa | ? | - |
? | |
3.2.1.186 | additional information | no activity amygdalin, cellobiose, gentiobiose, octyl beta-D-glucoside or prunasin | Hellenia speciosa | ? | - |
? | |
3.2.1.186 | protodioscin + H2O | - |
Hellenia speciosa | 26-deglucoprotodioscin + D-glucose | - |
? | |
3.2.1.186 | protogracillin | the enzyme is highly specific for cleavage of the C26-bound glucose moiety of furostanol glycosides | Hellenia speciosa | 26-deglucoprotogracillin + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.186 | dimer | 1 * 54000 + 1 * 58000, SDS-PAGE | Hellenia speciosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.186 | F26G | - |
Hellenia speciosa |
3.2.1.186 | furostanol 26-O-beta-D-glucoside glucohydrolase | - |
Hellenia speciosa |
3.2.1.186 | furostanol glycoside 26-O-beta-D-glucosidase | - |
Hellenia speciosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.186 | 40 | - |
assay at | Hellenia speciosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.186 | 5 | - |
assay at | Hellenia speciosa |
3.2.1.186 | 5 | 5.5 | substrate: protogracillin | Hellenia speciosa |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.186 | 1.4 | - |
pH 5.0, 40°C | Hellenia speciosa | beta-sitosterol | |
3.2.1.186 | 2.4 | - |
pH 5.0, 40°C | Hellenia speciosa | conduritol beta-epoxide | |
3.2.1.186 | 2.8 | - |
pH 5.0, 40°C | Hellenia speciosa | cholesterol |