EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.B26 | SDS | 50°C, 30 min, 0.03%, slight activation | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.B28 | expression in Escherichia coli | Pyrococcus furiosus |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.B26 | chaotropes (I-, ClO4-, NO3- and Cs+) have a strong destabilising effect on the protein by perturbing hydrophobic interactions | Saccharolobus solfataricus |
3.2.1.B26 | denaturing effect of NaCl and Na2SO4 | Saccharolobus solfataricus |
3.2.1.B28 | chaotropes (I-, ClO4-, NO3- and Cs+) have a strong destabilising effect on the protein by perturbing hydrophobic interactions | Pyrococcus furiosus |
3.2.1.B28 | denaturing effect of NaCl | Pyrococcus furiosus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.B26 | SDS | 50°C, 30 min, 0.3% SDS, 40% loss of activity | Saccharolobus solfataricus | |
3.2.1.B28 | SDS | 0.03%, 50% residual activity. 0.04% less than 10% residual activity | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | additional information | - |
additional information | KM increases with temperature: the increase is moderate at lower temperatures (from 30°C to 65°C) and more evident between 65°C and 85°C. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated | Saccharolobus solfataricus | |
3.2.1.B26 | 0.59 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 30°C | Saccharolobus solfataricus | |
3.2.1.B26 | 0.72 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 60°C | Saccharolobus solfataricus | |
3.2.1.B26 | 0.77 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 65°C | Saccharolobus solfataricus | |
3.2.1.B26 | 0.82 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 40°C | Saccharolobus solfataricus | |
3.2.1.B26 | 0.87 | - |
2-nitrophenyl beta-D-galactoside | pH 5.4, 75°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1.09 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 50°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1.61 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 75°C | Saccharolobus solfataricus | |
3.2.1.B26 | 3.16 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 85°C | Saccharolobus solfataricus | |
3.2.1.B28 | 0.25 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 0.37 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 1.11 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 30°C | Pyrococcus furiosus | |
3.2.1.B28 | 1.31 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 40°C | Pyrococcus furiosus | |
3.2.1.B28 | 1.97 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 65°C | Pyrococcus furiosus | |
3.2.1.B28 | 2.05 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 50°C | Pyrococcus furiosus | |
3.2.1.B28 | 2.09 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 60°C | Pyrococcus furiosus | |
3.2.1.B28 | 2.57 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 2.8 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 4.1 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 85°C | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B26 | Saccharolobus solfataricus | - |
- |
- |
3.2.1.B28 | Pyrococcus furiosus | Q51723 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B26 | - |
Saccharolobus solfataricus |
3.2.1.B28 | - |
Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B26 | 2-nitrophenyl beta-D-galactoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-galactoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.B26 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.B28 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.B28 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.B28 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
3.2.1.B28 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucopyranose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.B26 | Sbetagly | - |
Saccharolobus solfataricus |
3.2.1.B28 | CelB | - |
Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | additional information | - |
inactivation is coupled to irreversible aggregation | Saccharolobus solfataricus |
3.2.1.B26 | 75 | - |
30 min, 90% residual activity | Saccharolobus solfataricus |
3.2.1.B28 | 75 | - |
30 min, completely stable | Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B26 | additional information | - |
additional information | kcat increases exponentially with temperature. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated | Saccharolobus solfataricus | |
3.2.1.B26 | 56 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 30°C | Saccharolobus solfataricus | |
3.2.1.B26 | 143 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 40°C | Saccharolobus solfataricus | |
3.2.1.B26 | 293 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 50°C | Saccharolobus solfataricus | |
3.2.1.B26 | 648 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 60°C | Saccharolobus solfataricus | |
3.2.1.B26 | 1000 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 65°C | Saccharolobus solfataricus | |
3.2.1.B26 | 2288 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 75°C | Saccharolobus solfataricus | |
3.2.1.B26 | 2400 | - |
2-nitrophenyl beta-D-galactoside | pH 5.4, 75°C | Saccharolobus solfataricus | |
3.2.1.B26 | 4380 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 85°C | Saccharolobus solfataricus | |
3.2.1.B28 | 90 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 30°C | Pyrococcus furiosus | |
3.2.1.B28 | 236 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 40°C | Pyrococcus furiosus | |
3.2.1.B28 | 580 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 50°C | Pyrococcus furiosus | |
3.2.1.B28 | 1222 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 60°C | Pyrococcus furiosus | |
3.2.1.B28 | 1486 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 65°C | Pyrococcus furiosus | |
3.2.1.B28 | 2600 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 2907 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 3300 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B28 | 4863 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 85°C | Pyrococcus furiosus | |
3.2.1.B28 | 9600 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.4, 75°C | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 4.5 | 6.5 | at 65°C | Saccharolobus solfataricus |
3.2.1.B26 | 5 | 5.5 | at 75°C | Saccharolobus solfataricus |
3.2.1.B28 | 5.4 | - |
- |
Pyrococcus furiosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | additional information | - |
activity dependence on pH at 65°C and 75°C on 5 mM 4-nitrophenyl beta-D-galactoside | Saccharolobus solfataricus |
3.2.1.B26 | 4 | 7 | pH 4.0: about 60% of maximal activity at 75°C, pH 7.0: about 50% of maximal activity at 75°C | Saccharolobus solfataricus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B26 | 3.4 | - |
75°C, half-life is less than 5 min, irreversible aggregation | Saccharolobus solfataricus |
3.2.1.B28 | 10 | - |
75°C, 90 min, stable | Pyrococcus furiosus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 1000 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.21 | 3700 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.21 | 7100 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.21 | 12900 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.4, 75°C | Pyrococcus furiosus | |
3.2.1.B26 | additional information | - |
additional information | the efficiency (kcat/KM) increases with temperature and reaches its maximal efficiency at 65°C. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated | Saccharolobus solfataricus | |
3.2.1.B26 | 1400 | - |
4-nitrophenyl beta-D-galactoside | pH 5.4, 75°C | Saccharolobus solfataricus | |
3.2.1.B26 | 2700 | - |
2-nitrophenyl beta-D-galactoside | pH 5.4, 75°C | Saccharolobus solfataricus |