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Literature summary extracted from

  • Moessinger, J.; Wieffer, M.; Krause, E.; Freund, C.; Gerth, F.; Krauss, M.; Haucke, V.
    Phosphatidylinositol 4-kinase IIalpha function at endosomes is regulated by the ubiquitin ligase Itch (2012), EMBO Rep., 13, 1087-1094.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.67 expression of HA-tagged isozyme PI4KIIalpha in HEK-293 cells, co-expression of FLAG-tagged ubiquitin, PI4KIIalpha undergoes multi-ubiquitination Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.67 D308A enzymatically inactive isozyme PI4KIIalpha Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.67 endosome
-
 Homo sapiens 5768
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
2.7.1.67 additional information Homo sapiens E3 ubiquitin ligase Itch is a binding partner and regulator of PI4KIIalpha function. Itch directly associates with and ubiquitinates PI4KIIa ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.67 additional information PI4KIIalpha undergoes multi-ubiquitination via ubiquitin ligase Itch Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
2.7.1.67 additional information E3 ubiquitin ligase Itch is a binding partner and regulator of PI4KIIalpha function. Itch directly associates with and ubiquitinates PI4KIIa Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.67 phosphatidylinositol 4-kinase IIalpha
-
 Homo sapiens
2.7.1.67 PI 4-kinase type IIalpha
-
 Homo sapiens
2.7.1.67 PI4KIIalpha
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.67 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.67 malfunction depletion of PI4KIIalpha by siRNA-mediated knockdown reduces Wnt5a-triggered uptake and/or sorting of Fz4-eGFP into EEA1-positive endosomes. This effect is fully rescued by re-expression of siRNA-resistant PI4KIIalpha Homo sapiens
2.7.1.67 physiological function key role of isozyme PI4KIIalpha in TGN/endosomal membrane traffic, isozyme PI4KIIalpha regulates Wnt signalling, endosomal sorting of signalling receptors, and promotes adaptor protein recruitment to endosomes and the trans-Golgi network. E3 ubiquitin ligase Itch is a binding partner and regulator of PI4KIIa function. Itch directly associates with and ubiquitinates PI4KIIalpha, and both proteins co-localize on endosomes containing Wnt-activated frizzled 4 receptor. Itch and PI4KIIalpha reciprocally regulate each other Homo sapiens