Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, M.; Rao, Z.; Dou, W.; Jin, J.; Xu, Z.
    Site-directed mutagenesis studies on the L-arginine-binding sites of feedback inhibition in N-acetyl-L-glutamate kinase (NAGK) from Corynebacterium glutamicum (2012), Curr. Microbiol., 64, 164-172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.8 expressed in Escherichia coli as a His-tagged fusion protein Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.8 DELTA2-15 Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 71fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 DELTA2-19 Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 148fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 DELTA2-29 Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 163fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 DELTA25 Km (N-acetyl-L-glutamate) highly increased compared to wild-type, kcat highly decreased compared to wild-type, IC50 (L-arginine) 17fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 DELTA8 Km (N-acetyl-L-glutamate) increased compared to wild-type, kcat highly decreased compared to wild-type, IC50 (L-arginine) 16fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 E19A Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 40fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 E19R Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 61fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 E281A Km (N-acetyl-L-glutamate) highly increased compared to wild-type, kcat similar to wild-type, IC50 (L-arginine) 21fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 G287A Km (N-acetyl-L-glutamate) similar to wild-type, kcat decreased compared to wild-type, IC50 (L-arginine) 37fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 G287D Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 40fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 H268A Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 39fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 H268N Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 58fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 H26A Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 54fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 R209A Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 46fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 R209K Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 37fold increased compared to wild-type Corynebacterium glutamicum
2.7.2.8 W23A Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 4.5fold increased compared to wild-type Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 L-arginine
-
 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.8 3.14
-
 N-acetyl-L-glutamate mutant H26A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.25
-
 N-acetyl-L-glutamate mutant H268N, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.32
-
 N-acetyl-L-glutamate mutant G287D, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.43
-
 N-acetyl-L-glutamate mutant E19R, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.52
-
 N-acetyl-L-glutamate wild-type, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.61
-
 N-acetyl-L-glutamate mutant E19A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.72
-
 N-acetyl-L-glutamate mutant R209A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.76
-
 N-acetyl-L-glutamate mutant H268A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.86
-
 N-acetyl-L-glutamate mutant G287A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.89
-
 N-acetyl-L-glutamate mutant W23A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 3.96
-
 N-acetyl-L-glutamate mutant R209K, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 6.98
-
 N-acetyl-L-glutamate mutant DELTA8, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 7.84
-
 N-acetyl-L-glutamate mutant DELTA2-15, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 8.23
-
 N-acetyl-L-glutamate mutant DELTA2-19, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 8.45
-
 N-acetyl-L-glutamate mutant DELTA2-29, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 13.68
-
 N-acetyl-L-glutamate mutant E281A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 25.74
-
 N-acetyl-L-glutamate mutant DELTA25, pH and temperature not specified in the publication Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.8 36000
-
 SDS-PAGE Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8 using Ni-NTA chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Corynebacterium glutamicum ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.8 argB
-
 Corynebacterium glutamicum
2.7.2.8 NagK
-
 Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.8 2
-
 N-acetyl-L-glutamate mutant DELTA25, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 3.2
-
 N-acetyl-L-glutamate mutant DELTA25, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 4.2
-
 N-acetyl-L-glutamate mutant DELTA8, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 6.4
-
 N-acetyl-L-glutamate mutant DELTA8, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 7.2
-
 N-acetyl-L-glutamate mutant DELTA2-29, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 7.9
-
 N-acetyl-L-glutamate mutant DELTA2-29, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 11.5
-
 N-acetyl-L-glutamate mutant DELTA2-19, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 12.4
-
 N-acetyl-L-glutamate mutant DELTA2-19, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 13.8
-
 N-acetyl-L-glutamate mutant DELTA2-15, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 15.2
-
 N-acetyl-L-glutamate mutant DELTA2-15, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 31.4
-
 N-acetyl-L-glutamate wild-type, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 32.8
-
 N-acetyl-L-glutamate mutant W23A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 34.9
-
 N-acetyl-L-glutamate mutant E281A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 36.1
-
 N-acetyl-L-glutamate mutant G287A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 36.7
-
 N-acetyl-L-glutamate mutant G287A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 37.4
-
 N-acetyl-L-glutamate mutant R209K, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 38.3
-
 N-acetyl-L-glutamate mutant G287D, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 38.5
-
 N-acetyl-L-glutamate mutant E19A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 39.7
-
 N-acetyl-L-glutamate mutant R209A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 39.7
-
 N-acetyl-L-glutamate mutant W23A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 41
-
 N-acetyl-L-glutamate mutant E281A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 41.1
-
 N-acetyl-L-glutamate mutant H268A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 41.3
-
 N-acetyl-L-glutamate mutant G287D, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 41.9
-
 N-acetyl-L-glutamate mutant R209A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 42.1
-
 N-acetyl-L-glutamate mutant E19R, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 42.1
-
 N-acetyl-L-glutamate mutant R209K, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 43
-
 N-acetyl-L-glutamate mutant H26A, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 43.3
-
 N-acetyl-L-glutamate mutant H268N, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 43.4
-
 N-acetyl-L-glutamate mutant H26A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 43.8
-
 N-acetyl-L-glutamate mutant H268N, pH and temperature not specified in the publication, with 1 mM L-arginine Corynebacterium glutamicum
2.7.2.8 44.1
-
 N-acetyl-L-glutamate mutant E19A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 44.5
-
 N-acetyl-L-glutamate mutant H268A, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 44.8
-
 N-acetyl-L-glutamate mutant E19R, pH and temperature not specified in the publication Corynebacterium glutamicum
2.7.2.8 45.1
-
 N-acetyl-L-glutamate wild-type, pH and temperature not specified in the publication Corynebacterium glutamicum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.8 0.4
-
 wild-type, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 1.8
-
 mutant W23A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 6.4
-
 mutant DELTA8, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 6.8
-
 mutant DELTA25, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 8.6
-
 mutant E281A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 14.8
-
 mutant R209K, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 14.9
-
 mutant G287A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 15.7
-
 mutant H268A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 16.1
-
 mutant E19A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 16.2
-
 mutant G287D, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 18.5
-
 mutant R209A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 21.7
-
 mutant H26A, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 22.3
-
 mutant H268N, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 24.4
-
 mutant E19R, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 28.4
-
 mutant DELTA2-15, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 59.2
-
 mutant DELTA2-19, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine
2.7.2.8 65.2
-
 mutant DELTA2-29, pH and temperature not specified in the publication Corynebacterium glutamicum L-arginine