Any feedback?
Literature summary extracted from
- Purification and characterization of new special ginsenosidase hydrolyzing multi-glycosides of protopanaxadiol ginsenosides, ginsenosidase type I (2007), Chem. Pharm. Bull., 55, 231-235.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.193 | Cu2+ | - |
Aspergillus sp. |  |
| 3.2.1.193 | Pb2+ | - |
Aspergillus sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.193 | Ca2+ | 50-100 mM, slight activation | Aspergillus sp. | |
| 3.2.1.193 | Mg2+ | 50-100 mM, slight activation | Aspergillus sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 80000 | - |
x * 80000, SDS-PAGE | Aspergillus sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.193 | Aspergillus sp. | - |
- |
- |
| 3.2.1.193 | Aspergillus sp. g48p | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.193 | - |
Aspergillus sp. |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.193 | a protopanaxadiol-type ginsenoside with two glucosyl residues at position 3 + H2O = a protopanaxadiol-type ginsenoside with one glucosyl residue at position 3 + D-glucopyranose | Hydrolysis of the 3-O-beta-D-(1->2)-glucosidic bond, the 3-O-beta-D-glucopyranosyl bond and the 20-O-beta-D-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20 and no glucosyl or one glucosyl residue at position 3. Starting with a ginsenoside that is glycosylated at both positions (e.g. ginsenoside Rb1, Rb2, Rb3, Rc or Rd), the most common products are ginsenoside F2 and ginsenoside C-K with low amounts of ginsenoside Rh2. | Aspergillus sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 2.4 | - |
40°C, pH not specified in the publication, substrate: ginsenoside Rg3 | Aspergillus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.193 | ginsenoside Rb1 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside | Aspergillus sp. | ginsenoside F2 + 2 D-glucose | - |
? | |
| 3.2.1.193 | ginsenoside Rb1 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-glucoside | Aspergillus sp. g48p | ginsenoside F2 + 2 D-glucose | - |
? | |
| 3.2.1.193 | ginsenoside Rb2 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside | Aspergillus sp. | ginsenoside F2 + D-glucose + L-arabinopyranose | - |
? | |
| 3.2.1.193 | ginsenoside Rb2 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinopyranoside | Aspergillus sp. g48p | ginsenoside F2 + D-glucose + L-arabinopyranose | - |
? | |
| 3.2.1.193 | ginsenoside Rb3 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside | Aspergillus sp. | ginsenoside F2 + D-glucose + D-xylose | - |
? | |
| 3.2.1.193 | ginsenoside Rb3 + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-beta-D-(1->6)-xyloside | Aspergillus sp. g48p | ginsenoside F2 + D-glucose + D-xylose | - |
? | |
| 3.2.1.193 | ginsenoside Rc + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside | Aspergillus sp. | ginsenoside F2 + D-glucose + L-arabinofuranose | - |
? | |
| 3.2.1.193 | ginsenoside Rc + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside and 20-O-alpha-D-(1->6)-arabinofuranoside | Aspergillus sp. g48p | ginsenoside F2 + D-glucose + L-arabinofuranose | - |
? | |
| 3.2.1.193 | ginsenoside Rd + H2O | ginsenoside F2 is a main product plus ginsenoside C-K, produces small amounts of ginsenoside Rh2. Hydrolysis of 3-O-beta-D-(1->2)-glucoside | Aspergillus sp. | ginsenoside + D-glucose | - |
? | |
| 3.2.1.193 | additional information | the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 | Aspergillus sp. | ? | - |
? | |
| 3.2.1.193 | additional information | the enzyme can hydrolyze different glycoside of protopanaxadiol type ginsenosides, can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd, can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 | Aspergillus sp. g48p | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.193 | ? | x * 80000, SDS-PAGE | Aspergillus sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 40 | - |
assay at | Aspergillus sp. |
| 3.2.1.193 | 50 | - |
- |
Aspergillus sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 20 | 70 | 20°C: about 60% of maximal activity, 70°C: about 60% of maximal activity | Aspergillus sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 20 | 40 | 48 h, stable | Aspergillus sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 5 | - |
- |
Aspergillus sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 4 | 8 | pH 4.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity | Aspergillus sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.193 | 4.5 | 7 | stable | Aspergillus sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
