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Literature summary extracted from

  • Narikawa, T.; Shinoyama, H.; Fujii, T.
    A beta-rutinosidase from Penicillium rugulosum IFO 7242 that is a peculiar flavonoid glycosidase (2000), Biosci. Biotechnol. Biochem., 64, 1317-1379.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B31 65000
-
 x * 65000, SDS-PAGE Talaromyces rugulosus
3.2.1.B31 245000
-
 gel filtration Talaromyces rugulosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B31 Talaromyces rugulosus
-
-
-
3.2.1.B31 Talaromyces rugulosus IFO 7242
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B31 596
-
 pH and temperature not specified in the publication Talaromyces rugulosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B31 isoquercitrin + H2O i.e. quercetin 3-O-beta-D-glucoside, hydrolyzed at 98.7% compared to rutin Talaromyces rugulosus quercetin + beta-D-glucose
-
 ?
3.2.1.B31 isoquercitrin + H2O i.e. quercetin 3-O-beta-D-glucoside, hydrolyzed at 98.7% compared to rutin Talaromyces rugulosus IFO 7242 quercetin + beta-D-glucose
-
 ?
3.2.1.B31 additional information no activity with: quercitrin (i.e. quercetin 3-O-alpha-rhamnoside), hesperidin (i.e. hersperetin 7-O-beta-rutenoside), naringin (naringenin 7-O-beta-neohesperidoside), 4-nitrophenyl-alpha-rhamnoside, 4-nitrophenyl-beta-glucoside, methyl beta-glucoside, cellobiose Talaromyces rugulosus ?
-
 ?
3.2.1.B31 additional information no activity with: quercitrin (i.e. quercetin 3-O-alpha-rhamnoside), hesperidin (i.e. hersperetin 7-O-beta-rutenoside), naringin (naringenin 7-O-beta-neohesperidoside), 4-nitrophenyl-alpha-rhamnoside, 4-nitrophenyl-beta-glucoside, methyl beta-glucoside, cellobiose Talaromyces rugulosus IFO 7242 ?
-
 ?
3.2.1.B31 rutin + H2O i.e. quercetin 3-O-beta-rutenoside Talaromyces rugulosus quercetin + rutinose
-
 ?
3.2.1.B31 rutin + H2O i.e. quercetin 3-O-beta-rutenoside Talaromyces rugulosus IFO 7242 quercetin + rutinose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B31 ? x * 65000, SDS-PAGE Talaromyces rugulosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B31 beta-rutenosidase
-
 Talaromyces rugulosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B31 50
-
-
 Talaromyces rugulosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B31 40
-
 stable below Talaromyces rugulosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B31 2.2
-
-
 Talaromyces rugulosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B31 1.5 3 pH 1.5: about 50% of maximal activity (KCl-HCl buffer), pH 3.0: about 80% of maximal activity (glycine-HCl buffer) Talaromyces rugulosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B31 2 11 stable Talaromyces rugulosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.B31 Talaromyces rugulosus isoelectric focusing
-
 5