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Literature summary extracted from

  • Chung, S.K.; Ryu, S.I.; Lee, S.B.
    Characterization of UDP-glucose 4-epimerase from Pyrococcus horikoshii: regeneration of UDP to produce UDP-galactose using two-enzyme system with trehalose (2012), Biores. Technol., 110, 423-429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 expression in Escherichia coli Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.2 Cu2+ 5 mM, 95% loss of activity Pyrococcus horikoshii
5.1.3.2 Dimethylsulfoxide 10%, 26% inhibition Pyrococcus horikoshii
5.1.3.2 methanol 10%, 26% inhibition Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.2 0.23
-
 UDP-alpha-D-galactose pH 6.5, 60°C Pyrococcus horikoshii
5.1.3.2 2.24
-
 UDP-alpha-D-glucose pH 6.5, 60°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.2 Mn2+ 5 mM, activity is increased by 30% Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 35000
-
 2 * 35000, SDS-PAGE Pyrococcus horikoshii
5.1.3.2 70000
-
 gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Pyrococcus horikoshii O73960
-
-
5.1.3.2 Pyrococcus horikoshii OT-3 O73960
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-alpha-D-galactose
-
 Pyrococcus horikoshii UDP-alpha-D-glucose
-
 ?
5.1.3.2 UDP-alpha-D-galactose
-
 Pyrococcus horikoshii OT-3 UDP-alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.2 homodimer 2 * 35000, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 GalE
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.2 65
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.2 60
-
 1 h, stable up to Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.2 1
-
 UDP-alpha-D-galactose pH 6.5, 60°C Pyrococcus horikoshii
5.1.3.2 1.1
-
 UDP-alpha-D-glucose pH 6.5, 60°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.2 6.5
-
-
 Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.2 6 9 pH 6.0: about 80% of maximal activit, pH 9.0: about 50% of maximal activity Pyrococcus horikoshii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.2 5 8 highly stable Pyrococcus horikoshii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.2 0.49
-
 UDP-alpha-D-glucose pH 6.5, 60°C Pyrococcus horikoshii
5.1.3.2 4.34
-
 UDP-alpha-D-galactose pH 6.5, 60°C Pyrococcus horikoshii