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Literature summary extracted from

  • Zhang, Y.; Shang, X.; Deng, A.; Chai, X.; Lai, S.; Zhang, G.; Wen, T.
    Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine (2012), Biochimie, 94, 829-838.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 His-tagged protein expressed in Escherichia coli BL21(DE3) Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.17 A270P conserved residue Corynebacterium glutamicum
2.4.2.17 L231F/T235A conserved residues Corynebacterium glutamicum
2.4.2.17 N215K conserved residue Corynebacterium glutamicum
2.4.2.17 N215K/L231F/T235A conserved residues, 37fold increase in Ki-value of histidine Corynebacterium glutamicum
2.4.2.17 N215K/L231F/T235A/A270P conserved residues Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 ADP competitive to ATP Corynebacterium glutamicum
2.4.2.17 Ag+
-
 Corynebacterium glutamicum
2.4.2.17 AMP competitive to ATP Corynebacterium glutamicum
2.4.2.17 Ca2+ moderate inhibitory effect Corynebacterium glutamicum
2.4.2.17 Cu2+
-
 Corynebacterium glutamicum
2.4.2.17 EDTA moderate inhibitory effect Corynebacterium glutamicum
2.4.2.17 Fe2+
-
 Corynebacterium glutamicum
2.4.2.17 Fe3+
-
 Corynebacterium glutamicum
2.4.2.17 Hg2+ moderate inhibitory effect Corynebacterium glutamicum
2.4.2.17 L-histidine noncompetitive, alkaline pH decreases the inhibitory effect Corynebacterium glutamicum
2.4.2.17 Mn2+ moderate inhibitory effect Corynebacterium glutamicum
2.4.2.17 Zn2+ moderate inhibitory effect Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.07
-
 5-phospho-alpha-D-ribose 1-diphosphate A270P mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.08
-
 5-phospho-alpha-D-ribose 1-diphosphate N215K mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.08
-
 5-phospho-alpha-D-ribose 1-diphosphate N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.08
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.09
-
 5-phospho-alpha-D-ribose 1-diphosphate L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.22
-
 ATP N215K mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.22
-
 ATP wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.23
-
 ATP N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.24
-
 ATP A270P mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.35
-
 ATP L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+
-
 Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.17 32191
-
 2 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 Corynebacterium glutamicum
2.4.2.17 32191
-
 6 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 Corynebacterium glutamicum
2.4.2.17 63000
-
 homodimer, gel filtration Corynebacterium glutamicum
2.4.2.17 192000
-
 homohexamer, gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Corynebacterium glutamicum Q9Z472
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 immobilized metal ion affinity chromatography, gel filtration Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.17 0.39
-
 N215K/L231F/T235A/A270P mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 1.96
-
 A270P mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 2.01
-
 N215K mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 2.04
-
 L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 2.1
-
 N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 2.19
-
 wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Corynebacterium glutamicum 1-(5-phospho-alpha-D-ribosyl)-ATP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.17 homodimer 2 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 Corynebacterium glutamicum
2.4.2.17 homohexamer 6 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 ATP phosphoribosyltransferase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.17 30
-
-
 Corynebacterium glutamicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.17 20 50
-
 Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.17 1.29
-
 5-phospho-alpha-D-ribose 1-diphosphate A270P mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 1.36
-
 5-phospho-alpha-D-ribose 1-diphosphate L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 1.75
-
 5-phospho-alpha-D-ribose 1-diphosphate N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 1.91
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 2.22
-
 5-phospho-alpha-D-ribose 1-diphosphate N215K mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.17 10
-
-
 Corynebacterium glutamicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.17 7.5 13 >40% relative activity, rapid loss of activity below pH 7.5 Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.17 0.11
-
 L-histidine wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.24
-
 L-histidine A270P mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.28
-
 L-histidine N215K mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.52
-
 L-histidine L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 0.88
-
 ADP wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 1.29
-
 AMP wild type protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum
2.4.2.17 4.15
-
 L-histidine N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
2.4.2.17 metabolism essential for histidine biosynthesis Corynebacterium glutamicum