EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.1 | expression in Escherichia coli | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.1 | crystal structures of the wild type in complexes with phosphate and sulfate, respectively, and of the R24A mutant in complex with phosphate/sulfate, to about 2 A resolution. The structural data show that previously observed conformational change is a result of the phosphate binding and its interaction with residue Arg24 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.1 | D204A | 0.1% of wild-type activity towards inosine, adenosine and guanosine | Escherichia coli |
2.4.2.1 | D204A/R217A | about 0.2% of wild-type activity towards inosine, adenosine and guanosine | Escherichia coli |
2.4.2.1 | D204N | less than about 4% of wild-type activity towards inosine, adenosine and guanosine | Escherichia coli |
2.4.2.1 | R217A | about 0.5% of wild-type activity towards inosine and guanosine, 10.8% of activity towards adenosine | Escherichia coli |
2.4.2.1 | R24A | absence of a conformational change upon binding of phosphate, about 0.2 to 0.6% of wild-type activity towards inosine, adenosine and guanosine | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.1 | Escherichia coli | P0ABP8 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.1 | 0.041 | - |
substrate inosine, mutant R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.05 | - |
substrate adenosine, mutant D204A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.06 | - |
substrate guanosine, mutant D204A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.07 | - |
substrate adenosine, mutant D204N, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.07 | - |
substrate guanosine, mutant D204A/R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.09 | - |
substrate guanosine, mutant R24A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.09 | - |
substrate inosine, mutant D204A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.13 | - |
substrate adenosine, mutant D204A/R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.14 | - |
substrate inosine, mutant D204A/R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.18 | - |
substrate inosine, mutant R24A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.36 | - |
substrate adenosine, mutant R24A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 0.36 | - |
substrate guanosine, mutant R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 1.4 | - |
substrate inosine, mutant D204N, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 2.1 | - |
substrate guanosine, mutant D204N, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 6.3 | - |
substrate adenosine, mutant R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 6.4 | - |
substrate 7-methylguanosine, mutant R24A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 6.9 | - |
substrate 7-methylguanosine, mutant D204N, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 20 | - |
substrate 7-methylguanosine, mutant D204A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 21.3 | - |
substrate 7-methylguanosine, mutant R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 21.4 | - |
substrate 7-methylguanosine, wild-type, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 46.9 | - |
substrate 7-methylguanosine, mutant D204A/R217A, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 58.5 | - |
substrate adenosine, wild-type, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 60.4 | - |
substrate guanosine, wild-type, pH 7.0, 25°C | Escherichia coli |
2.4.2.1 | 103.2 | - |
substrate inosine, wild-type, pH 7.0, 25°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.1 | 7-methylguanosine + phosphate | - |
Escherichia coli | 7-methylguanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | adenosine + phosphate | - |
Escherichia coli | adenine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | guanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.1 | additional information | catalysis involves protonation of the purine base at position N7 by residue Asp204, which is triggered by Arg217 | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.1 | DeoD | - |
Escherichia coli |