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Literature summary extracted from

  • He, S.M.; Wathier, M.; Podzelinska, K.; Wong, M.; McSorley, F.R.; Asfaw, A.; Hove-Jensen, B.; Jia, Z.; Zechel, D.L.
    Structure and mechanism of PhnP, a phosphodiesterase of the carbon-phosphorus lyase pathway (2011), Biochemistry, 50, 8603-8615.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.55 complexed with orthovanadate to 1.5 A resolution. Orthovanadate binds in a tetrahedral geometry by the two catalytic manganese ions and the putative general acid residue H200 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.55 D164A 0.07% of wild-type activity Escherichia coli
3.1.4.55 D187A 83% of wild-type activity Escherichia coli
3.1.4.55 D54A 0.09% of wild-type activity Escherichia coli
3.1.4.55 D80A 0.07% of wild-type activity Escherichia coli
3.1.4.55 H200A 10% of wild-type activity, 6.33fold decrease in kcat/KM with substituted methyl phenylphosphate diesters with leaving group pKa values ranging from 4 to 8.4. With bis(pnitrophenyl)phosphate as a substrate, there is a 10fold decrease in kcat/KM, primarily the result of a large increase in KM. The nickel ion-activated H200A mutant displays a bell-shaped pH dependence for kcat/KM with pKa values comparable to those of the wild-type enzyme Escherichia coli
3.1.4.55 H222A 14% of wild-type activity Escherichia coli
3.1.4.55 H78A 0.3% of wild-type activity Escherichia coli
3.1.4.55 T75A 3% of wild-type activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.55 orthovanadate crystallization data Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.55 2.4
-
 bis(p-nitrophenyl)phosphate mutant D54A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 3
-
 bis(p-nitrophenyl)phosphate mutant H222A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 3.4
-
 bis(p-nitrophenyl)phosphate mutant D187A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 3.5
-
 bis(p-nitrophenyl)phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 3.7
-
 bis(p-nitrophenyl)phosphate mutant D164A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 3.7
-
 bis(p-nitrophenyl)phosphate mutant D80A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 3.8
-
 bis(p-nitrophenyl)phosphate mutant H78A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 18
-
 bis(p-nitrophenyl)phosphate mutant H200A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 28
-
 bis(p-nitrophenyl)phosphate mutant T75A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.55 Mn2+ inhibitor orthovanadate binds in a tetrahedral geometry by the two catalytic manganese ions and the putative general acid residue H200 Escherichia coli
3.1.4.55 Ni2+ may substitute for Mn2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.55 Escherichia coli P16692
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.55 bis(p-nitrophenyl)phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 2,4-dichlorophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 2,4-dinitrophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 2,5-dinitrophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 2-chloro-4-nitrophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 3-nitrophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 4-chlorophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 4-cyanophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl 4-nitrophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl nitrophenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.4.55 methyl phenyl phosphate + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.55 phnP
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.55 0.001
-
 bis(p-nitrophenyl)phosphate mutant D164A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.001
-
 bis(p-nitrophenyl)phosphate mutant D54A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.001
-
 bis(p-nitrophenyl)phosphate mutant D80A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.003
-
 bis(p-nitrophenyl)phosphate mutant H78A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.13
-
 bis(p-nitrophenyl)phosphate mutant H222A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.25
-
 bis(p-nitrophenyl)phosphate mutant T75A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.55
-
 bis(p-nitrophenyl)phosphate mutant H200A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.88
-
 bis(p-nitrophenyl)phosphate mutant D187A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 1.07
-
 bis(p-nitrophenyl)phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.55 7
-
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.55 0.001
-
 bis(p-nitrophenyl)phosphate mutant H78A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.0024
-
 methyl 4-chlorophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.006
-
 methyl 4-cyanophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.009
-
 bis(p-nitrophenyl)phosphate mutant T75A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.009
-
 methyl phenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.03
-
 bis(p-nitrophenyl)phosphate mutant H200A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.041
-
 methyl 3-nitrophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.043
-
 bis(p-nitrophenyl)phosphate mutant H222A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.047
-
 methyl 4-nitrophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.124
-
 methyl 2,4-dichlorophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.22
-
 methyl 2,4-dinitrophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.25
-
 bis(p-nitrophenyl)phosphate mutant D187A, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.29
-
 methyl 2-chloro-4-nitrophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.3
-
 bis(p-nitrophenyl)phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli
3.1.4.55 0.68
-
 methyl 2,5-dinitrophenyl phosphate wild-type, pH 7.1, 25°C, presence of Mn2+ Escherichia coli