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Literature summary extracted from

  • Cook, P.D.; Kubiak, R.L.; Toomey, D.P.; Holden, H.M.
    Two site-directed mutations are required for the conversion of a sugar dehydratase into an aminotransferase (2009), Biochemistry, 48, 5246-5253.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.102 K186H single mutant enzyme has no detectable catalytic activity with its sugar substrate Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.102 0.013
-
 GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 7.5, 22°C Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.102 GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate Caulobacter vibrioides
-
 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?
2.6.1.102 GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate Caulobacter vibrioides CB15
-
 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.102 Caulobacter vibrioides
-
-
-
2.6.1.102 Caulobacter vibrioides CB15
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.102 GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate
-
 Caulobacter vibrioides GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?
2.6.1.102 GDP-4-dehydro-6-deoxy-alpha-D-mannose + L-glutamate
-
 Caulobacter vibrioides CB15 GDP-4-amino-4,6-dideoxy-alpha-D-mannose + 2-oxoglutarate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.102 GDP-perosamine synthase
-
 Caulobacter vibrioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.102 2.7
-
 GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 7.5, 22°C Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.102 pyridoxal 5'-phosphate pyridoxal 5'-phosphate-dependent enzyme Caulobacter vibrioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.102 208
-
 GDP-4-dehydro-6-deoxy-alpha-D-mannose pH 7.5, 22°C Caulobacter vibrioides