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Literature summary extracted from

  • Lehmann, C.; Begley, T.P.; Ealick, S.E.
    Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis (2006), Biochemistry, 45, 11-19.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.73 expressed in Escherichia coli BL834(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.73 in complex with ThiS, hanging drop vapor diffusion method, using 7-8% polyethylene glycol 400, 35 mM calcium chloride, and 100 mM Tris-HCl (pH 7.0-7.3) Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.73 Zn2+ ThiF contains a Zn-sulfur center Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.73 27000
-
 2 * 27000, calculated from amino acid sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.73 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.73 Hi-Trap QFF column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.73 ATP + [sulfur-carrier protein ThiS] ThiF catalyzes the adenylation of the carboxy terminus of ThiS and the subsequent displacement of AMP catalyzed by ThiI-persulfide to give a ThiS-ThiI acyl disulfide Escherichia coli diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.73 homodimer 2 * 27000, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.7.73 ThiF
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.73 ATP
-
 Escherichia coli