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Literature summary extracted from

  • Gerratana, B.; Arnett, S.O.; Stapon, A.; Townsend, C.A.
    Carboxymethylproline synthase from Pectobacterium carotorova: a multifaceted member of the crotonase superfamily (2004), Biochemistry, 43, 15936-15945.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.226
-
 Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.226 0.0015
-
 (S)-1-pyrroline-5-carboxylate pH 7.8, 22°C Pectobacterium carotovorum
2.3.1.226 0.0027
-
 malonyl-CoA pH 7.8, 22°C Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O Pectobacterium carotovorum
-
 CoA + (2S,5S)-carboxymethylproline + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.226 Pectobacterium carotovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.226
-
 Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O
-
 Pectobacterium carotovorum CoA + (2S,5S)-5-carboxymethylproline + CO2
-
 ?
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O
-
 Pectobacterium carotovorum CoA + (2S,5S)-carboxymethylproline + CO2
-
 ?
2.3.1.226 methylmalonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O
-
 Pectobacterium carotovorum CoA + (2S,5S)-6-methyl-5-carboxymethylproline + CO2
-
 ?
2.3.1.226 additional information CarB catalyzes the independent decarboxylation of malonyl-CoA and methylmalonyl-CoA and the hydrolysis of CoA esters such as acetyl-CoA and propionyl-CoA Pectobacterium carotovorum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.226 CarB
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.226 22
-
 assay at Pectobacterium carotovorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.226 1.52
-
 (S)-1-pyrroline-5-carboxylate pH 7.8, 22°C Pectobacterium carotovorum
2.3.1.226 1.7
-
 malonyl-CoA pH 7.8, 22°C Pectobacterium carotovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.226 7.8
-
 assay at Pectobacterium carotovorum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.226 630
-
 malonyl-CoA pH 7.8, 22°C Pectobacterium carotovorum
2.3.1.226 984
-
 (S)-1-pyrroline-5-carboxylate pH 7.8, 22°C Pectobacterium carotovorum